Anti-Thyroid Peroxidase/TPO 抗体 [EPR5380] (ab109383)
![Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)](https://www.abcam.co.jp/ps/products/109/ab109383/Images/ab109383-122172-anti-thyroid-peroxidase-tpo-antibody-epr5380-western-blot.jpg "Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5380] to Thyroid Peroxidase/TPO
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Thyroid Peroxidase/TPO antibody [EPR5380]
Thyroid Peroxidase/TPO 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5380] to Thyroid Peroxidase/TPO -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human thyroid lysate IHC-P: Human thyroid gland tissue IP: Human thyroid cell lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5380 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109383の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 103 kDa.
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| IP |
1/10 - 1/100.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 103 kDa. |
|
IP
1/10 - 1/100. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
ターゲット情報
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機能
Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). -
パスウェイ
Hormone biosynthesis; thyroid hormone biosynthesis. -
関連疾患
Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. -
配列類似性
Belongs to the peroxidase family. XPO subfamily.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain. -
翻訳後修飾
Glycosylated.
Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
Cleaved in its N-terminal part. -
細胞内局在
Membrane and Cell surface. - Information by UniProt
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参照データベース
- Entrez Gene: 7173 Human
- Omim: 606765 Human
- SwissProt: P07202 Human
- Unigene: 467554 Human
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別名
- MSA antibody
- PERT_HUMAN antibody
- TDH2A antibody
see all
画像
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Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383) at 1/1000 dilution + Human thyroid lysate at 10 µg
Predicted band size: 103 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)](https://www.abcam.co.jp/ps/products/109/ab109383/Images/ab109383-122171-anti-thyroid-peroxidase-tpo-antibody-epr5380-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)ab109383, at a 1/100 dilution, staining Thyroid Peroxidase/TPO in paraffin-embedded Human thyroid gland tissue
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)](https://www.abcam.co.jp/ps/products/109/ab109383/Images/ab109383-4-anti-thyroid-peroxidase-tpo-antibody-epr5380-immunoprecipitation-thyroid-human.jpg)
Immunoprecipitation - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)Thyroid Peroxidase/TPO was immunoprecipitated from 0.35 mg Human thyroid whole cell lysate 10 µg with 109383 at 1/100 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human thyroid whole cell lysate 10 µg
Lane 2: ab109383 IP in Human thyroid whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109383 in Human thyroid whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)](https://www.abcam.co.jp/ps/products/109/ab109383/Images/ab109383-3-benefits-of-recombinant-antibodies.png)
Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab109383 は 5 報の論文で使用されています。
- Zabegina L et al. MiRNA let-7 from TPO(+) Extracellular Vesicles is a Potential Marker for a Differential Diagnosis of Follicular Thyroid Nodules. Cells 9:N/A (2020). PubMed: 32824820
- Yakou F et al. A Novel Homozygous Mutation of Thyroid Peroxidase Gene Abolishes a Disulfide Bond Leading to Congenital Hypothyroidism. Int J Endocrinol 2020:9132372 (2020). PubMed: 32908504
- Zane M et al. Estrogen and thyroid cancer is a stem affair: A preliminary study. Biomed Pharmacother 85:399-411 (2017). ICC/IF . PubMed: 27899250
- Narumi S et al. Mild thyroid peroxidase deficiency caused by TPO mutations with residual activity: Correlation between clinical phenotypes and enzymatic activity. Endocr J 64:1087-1097 (2017). PubMed: 28867693
- Fernando R et al. Expression of thyrotropin receptor, thyroglobulin, sodium-iodide symporter, and thyroperoxidase by fibrocytes depends on AIRE. J Clin Endocrinol Metab 99:E1236-44 (2014). PubMed: 24708100
