Application
Other - Do not use
Sample
Human Cell lysate - nuclear (WI38 cell line)
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: ECL Mouse IgG, HRP-linked F(ab')2 fragment (from s
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Notes
DNA dot-blot:
Genomic DNA was isolated from WI38 cells untreated (0h) or treated with 150J/m2 UVC (1h and 2h after treatment). Samples were denaturated at 95oC for 10 min and placed on ice for 5 min. 25ng of genomic DNA was spotted onto Hybond N+ membrane. The membrane was allowed to air dry. The membrane was placed in a box with damp paper towels and DNA was twice autocrosslinked in a Stratalinker UV crosslinker (model 1800). The membrane was allowed to air dry and then blocked for 1h in 5% milk in TBS. The membrane was probed with primary antibody solution in 5% milk in TBS for 1h at room temperature, washed three times for 10 min with TTBS, probed with secondary antibody solution in 5% milk in TBS for 1h at room temperature and washed three times for 10 min with TTBS. Then the membrane was developed using ECL method.
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投稿 Dec 08 2017