Anti-Thymine Dimer 抗体 [H3] (ab10347)
![Immunocytochemistry/ Immunofluorescence - Anti-Thymine Dimer antibody [H3] (ab10347)](https://www.abcam.co.jp/ps/products/10/ab10347/Images/ab10347-17951-anti-thymine-dimer-antibody-h3-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Thymine Dimer antibody [H3] (ab10347)")
Key features and details
- Mouse monoclonal [H3] to Thymine Dimer
- Suitable for: Southern Blot, ICC, Competitive ELISA, ELISA, ICC/IF
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-Thymine Dimer antibody [H3] -
製品の詳細
Mouse monoclonal [H3] to Thymine Dimer -
由来種
Mouse -
アプリケーション
適用あり: Southern Blot, ICC, Competitive ELISA, ELISA, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule.
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ポジティブ･コントロール
- ICC/IF: HeLa cells.
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特記事項
Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromato-graphic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induction and repair of these lesions without the requirement of in vivo radiolabeling of DNA. Photoimmunodetection, is a rapid, reliable and low-cost supplement to existing methods for nonradioactive DNA labeling. It enables a sensitive and non-radioactive method for labeling, detection, and quantification of high molecular weight (HMW) DNA fragments. The method is based on the introduction of thymine dimers into DNA after separa-tion by pulse field gel electrophoresis (PFGE), followed by detection with thymine dimer specific antibodies. The method does not require any enzymatic or chemical manipulation of the DNA sample. Monoclonal anti-bodies reacting specifically with thymine dimer, facilitate investigations on the apoptotic process and the role of UV-induced pyrimidine dimers in the process of photocarcinogenesis.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
一次抗体 備考
Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromato-graphic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induction and repair of these lesions without the requirement of in vivo radiolabeling of DNA. Photoimmunodetection, is a rapid, reliable and low-cost supplement to existing methods for nonradioactive DNA labeling. It enables a sensitive and non-radioactive method for labeling, detection, and quantification of high molecular weight (HMW) DNA fragments. The method is based on the introduction of thymine dimers into DNA after separa-tion by pulse field gel electrophoresis (PFGE), followed by detection with thymine dimer specific antibodies. The method does not require any enzymatic or chemical manipulation of the DNA sample. Monoclonal anti-bodies reacting specifically with thymine dimer, facilitate investigations on the apoptotic process and the role of UV-induced pyrimidine dimers in the process of photocarcinogenesis. -
ポリ/モノ
モノクローナル -
クローン名
H3 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10347の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Southern Blot |
Use a concentration of 0.5 - 1 µg/ml.
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| ICC |
Use at an assay dependent dilution.
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| Competitive ELISA |
Use at an assay dependent dilution.
|
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| ELISA | (1) |
Use at an assay dependent dilution.
|
| ICC/IF | (2) |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Southern Blot
Use a concentration of 0.5 - 1 µg/ml. |
|
ICC
Use at an assay dependent dilution. |
|
Competitive ELISA
Use at an assay dependent dilution. |
|
ELISA
Use at an assay dependent dilution. |
|
ICC/IF
Use at an assay dependent concentration. |
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Thymine Dimer antibody [H3] (ab10347)This image is courtesy of an anonymous Abreview.ab10347 staining Thymine Dimer in HeLa cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in formaldehyde, permabilized using 0.5% Triton X-100, blocked with 5% BSA for 15 minutes at 20°C, then incubated with ab10347 at a 1/250 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated rabbit anti mouse polyclonal, used at a 1/500 dilution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab10347 は 11 報の論文で使用されています。
- Tedeschi F et al. EFFECTOR OF TRANSCRIPTION factors are novel plant-specific regulators associated with genomic DNA methylation in Arabidopsis. New Phytol 221:261-278 (2019). PubMed: 30252137
- Yadav VK et al. Detection of UV-Induced Thymine Dimers. Methods Mol Biol 2031:313-322 (2019). PubMed: 31473968
- Boisnic S et al. Anti-inflammatory and antiradical effects of a 2% diosmin cream in a human skin organ culture as model. J Cosmet Dermatol 17:848-854 (2018). PubMed: 30203575
- Cobb AM et al. Disruption of PCNA-lamins A/C interactions by prelamin A induces DNA replication fork stalling. Nucleus 7:498-511 (2016). PubMed: 27676213
- Torregrosa-Muñumer R et al. Low doses of ultraviolet radiation and oxidative damage induce dramatic accumulation of mitochondrial DNA replication intermediates, fork regression, and replication initiation shift. Mol Biol Cell 26:4197-208 (2015). PubMed: 26399294