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リコンビナントRabMAb

Anti-Thymidylate Synthase 抗体 [EPR4545] (ab108995)

Reviews (2) Submit a question References (12)
Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
  • Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
  • Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
  • Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
  • Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
  • Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4545] to Thymidylate Synthase
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 APC Carrier Free PE

製品の概要

  • 製品名

    Anti-Thymidylate Synthase antibody [EPR4545]
    Thymidylate Synthase 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4545] to Thymidylate Synthase

  • 由来種

    Rabbit

  • 特異性

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, Jurkat, HL60 and MCF7 cell lysates; Human ovary carcinoma and colon carcinoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108995の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IP
1/10 - 1/100.
IHC-P (1)
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 minutes.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
ICC/IF
1/100 - 1/250.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 minutes.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
ICC/IF
1/100 - 1/250.

ターゲット情報

画像

  • Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
    Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
    All lanes : Anti-Thymidylate Synthase antibody [EPR4545] (ab108995) at 1/10000 dilution (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
    Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 4 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
    Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)

    ab108995 (purified) at 1:30 dilution (2μg) immunoprecipitating Thymidylate Synthase in Jurkat whole cell lysate.

    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate,10μg
    Lane 2 (+): ab108995 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108995 in Jurkat whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
    Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Thymidylate Synthase with purified ab108995 at 1/50 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
    Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Thymidylate Synthas with Purified ab108995 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100 ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling Thymidylate Synthase with purified ab108995 at 1:100 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
    Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
    All lanes : Anti-Thymidylate Synthase antibody [EPR4545] (ab108995) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HL60 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)

    Unpurified ab108995 at 1/50 dilution, staining Thymidylate Synthase in Human ovary carcinoma Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
    Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)

参考文献 (12)

ab108995 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108995 は 12 報の論文で使用されています。

  • Wang D  et al. Tumor mutation burden as a biomarker in resected gastric cancer via its association with immune infiltration and hypoxia. Gastric Cancer 24:823-834 (2021). PubMed: 33687617
  • Shorstova T  et al. Reprogramming of Nucleotide Metabolism Mediates Synergy between Epigenetic Therapy and MAP Kinase Inhibition. Mol Cancer Ther 20:64-75 (2021). PubMed: 33087508
  • Tao Q  et al. Perineural Invasion and Postoperative Adjuvant Chemotherapy Efficacy in Patients With Gastric Cancer. Front Oncol 10:530 (2020). PubMed: 32373527
  • Zhang S  et al. Identification of TYMS as a promoting factor of retroperitoneal liposarcoma progression: Bioinformatics analysis and biological evidence. Oncol Rep 44:565-576 (2020). PubMed: 32627015
  • Duan R  et al. Tumor Microenvironment Status Predicts the Efficacy of Postoperative Chemotherapy or Radiochemotherapy in Resected Gastric Cancer. Front Immunol 11:609337 (2020). PubMed: 33569057
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

IHC - Wholemount abreview for Anti-Thymidylate Synthase antibody [EPR4545]

 Good
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (Cornea)
Specification
Cornea
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 14 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Thymidylate Synthase antibody [EPR4545]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon adenocarcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 8.5 buffer
Permeabilization
No
Specification
Colon adenocarcinoma
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Allan Wang

Verified customer

投稿 Mar 20 2018

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