Anti-Thymidylate Synthase 抗体 (ab7398)

Submit a review Q&A (6)References (7)

Key features and details

  • Sheep polyclonal to Thymidylate Synthase
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Escherichia coli, African green monkey
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Thymidylate Synthase antibody
    Thymidylate Synthase 一次抗体 製品一覧

  • 製品の詳細

    Sheep polyclonal to Thymidylate Synthase

  • 由来種

    Sheep

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human, Escherichia coli, African green monkey
    非交差種: Saccharomyces cerevisiae

  • 免疫原

    Recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.

  • 特記事項

    The antibody recognizes the expected additional band corresponding to the ternary complex of human TS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes 36 kDa human TS.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.

ターゲット情報

画像

  • Western blot - Anti-Thymidylate Synthase antibody (ab7398)
    Western blot - Anti-Thymidylate Synthase antibody (ab7398)
    ab7398 is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 µg of protein. Lanes 1, 2 and 3 represent 1/2000, 1/5000 and 1/10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG diluted 1/1000 and color development using TMB substrate for approximately 4'.

参考文献 (7)

ab7398 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7398 は 7 報の論文で使用されています。

  • Zhang L  et al. Disrupted folate metabolism with anesthesia leads to myelination deficits mediated by epigenetic regulation of ERMN. EBioMedicine 43:473-486 (2019). PubMed: 31060905
  • Gu YJ  et al. GRP78 confers the resistance to 5-FU by activating the c-Src/LSF/TS axis in hepatocellular carcinoma. Oncotarget 6:33658-74 (2015). WB . PubMed: 26378040
  • MacFarlane AJ  et al. Azoxymethane-induced colon carcinogenesis in mice occurs independently of de novo thymidylate synthesis capacity. J Nutr 144:419-24 (2014). PubMed: 24500934
  • Macfarlane AJ  et al. Nuclear localization of de novo thymidylate biosynthesis pathway is required to prevent uracil accumulation in DNA. J Biol Chem 286:44015-22 (2011). WB ; Mouse . PubMed: 22057276
  • Gay S  et al. Nucleotide supply, not local histone acetylation, sets replication origin usage in transcribed regions. EMBO Rep 11:698-704 (2010). WB ; Chinese hamster . PubMed: 20671737
  • Fox JT  et al. A UV-responsive internal ribosome entry site enhances serine hydroxymethyltransferase 1 expression for DNA damage repair. J Biol Chem 284:31097-108 (2009). WB, IP ; Human . PubMed: 19734144
  • Woeller CF  et al. Evidence for small ubiquitin-like modifier-dependent nuclear import of the thymidylate biosynthesis pathway. J Biol Chem 282:17623-31 (2007). PubMed: 17446168

レビューと Q&A

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1-6 of 6 Abreviews or Q&A

Question

Thank you very much for Proforma invoice. We will inform our customer and after then we will send an official order. But Hthe customer would like to know, if POSITIVE CINTROL is possible to buy from Abcam.

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Answer

Thank you for your enquiry. For ab7398 and 3145 - you can use 5-FU-resistant cancer cell lines or human breast, colorectal, gastric, head and neck carcinomas as positive controls. All the cells especially the rapidly proliferating cells express this enzyme. For details of our human tumor cell lysate, please take a look at our on-line catalogue: https://www.abcam.com/index.html?pageconfig=catalog_byproducttype&intProductTypeID=12&intProductSubTypeID=3

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Question

Please would you let me know the subclass of the antibody to thymidylate synthase and whether the light chain is kappa or lambda. Also, what is the sensitivity of the antibody.

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Answer

This is a polyclonal antibody, and so we cannot refer to subclasses. The concentration is 10mg/ml and the sensitivity can be seen from the recommended concentrations for each application.

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Question

Is the antibody directed to the N-terminal?

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Answer

This antibody was raised against the full length recombinant protien. The epitopes recognised by this antibody have not been mapped.

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Question

Would you please let me know what is the carrier protein for the antibody.

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Answer

This antibody was raised against a recombinant protein. A carrier protein was not used for immunisation.

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Question

Do you have an example of the results obtained for a Western Blot for this antibody. Also the quote is for 1 mg of antibody. Is this 1 mg of an IgG fraction or for purified antibody?

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Answer

The antibody is an IgG fraction. Unfortunatly we do not have an example to hand of the Western blot. However, the antibody works very well in Western blotting and we recommend this antibody for this application.

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Question

Does your antibody (THymidylate Synthase) work on paraffin?

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Answer

Yes, the anti-hTS does work on paraffin.

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