Anti-Thymidylate Synthase 抗体 (ab7398)

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Western blot - Anti-Thymidylate Synthase antibody (ab7398)

    Key features and details

    • Sheep polyclonal to Thymidylate Synthase
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Thymidylate Synthase antibody
      Thymidylate Synthase 一次抗体 製品一覧

    • 製品の詳細

      Sheep polyclonal to Thymidylate Synthase

    • 由来種

      Sheep

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      非交差種: Saccharomyces cerevisiae

    • 免疫原

      Recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.

    • 特記事項

      The antibody recognizes the expected additional band corresponding to the ternary complex of human TS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes 36 kDa human TS.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab7398の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
    特記事項
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.

    ターゲット情報

    • 機能

      Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.

    • パスウェイ

      Pyrimidine metabolism; dTTP biosynthesis.

    • 配列類似性

      Belongs to the thymidylate synthase family.

    • 細胞内局在

      Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • d TMP synthase antibody
        • EC 2.1.1.45 antibody
        • HsT422 antibody
        • MGC88736 antibody
        • OTTHUMP00000162195 antibody
        • Thymidylate synthase antibody
        • Thymidylate synthetase antibody
        • TMS antibody
        • TS antibody
        • TSase antibody
        • Tyms antibody
        • TYMS protein antibody
        • Tyms thymidylate synthetase antibody
        • TYSY_HUMAN antibody
        see all

      画像

      • Western blot - Anti-Thymidylate Synthase antibody (ab7398)
        Western blot - Anti-Thymidylate Synthase antibody (ab7398)
        ab7398 is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 µg of protein. Lanes 1, 2 and 3 represent 1/2000, 1/5000 and 1/10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG diluted 1/1000 and color development using TMB substrate for approximately 4'.

      参考文献 (7)

      ab7398 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab7398 は 7 報の論文で使用されています。

      • Zhang L  et al. Disrupted folate metabolism with anesthesia leads to myelination deficits mediated by epigenetic regulation of ERMN. EBioMedicine 43:473-486 (2019). PubMed: 31060905
      • Gu YJ  et al. GRP78 confers the resistance to 5-FU by activating the c-Src/LSF/TS axis in hepatocellular carcinoma. Oncotarget 6:33658-74 (2015). WB . PubMed: 26378040
      • MacFarlane AJ  et al. Azoxymethane-induced colon carcinogenesis in mice occurs independently of de novo thymidylate synthesis capacity. J Nutr 144:419-24 (2014). PubMed: 24500934
      • Macfarlane AJ  et al. Nuclear localization of de novo thymidylate biosynthesis pathway is required to prevent uracil accumulation in DNA. J Biol Chem 286:44015-22 (2011). WB ; Mouse . PubMed: 22057276
      • Gay S  et al. Nucleotide supply, not local histone acetylation, sets replication origin usage in transcribed regions. EMBO Rep 11:698-704 (2010). WB ; Chinese hamster . PubMed: 20671737
      • Fox JT  et al. A UV-responsive internal ribosome entry site enhances serine hydroxymethyltransferase 1 expression for DNA damage repair. J Biol Chem 284:31097-108 (2009). WB, IP ; Human . PubMed: 19734144
      • Woeller CF  et al. Evidence for small ubiquitin-like modifier-dependent nuclear import of the thymidylate biosynthesis pathway. J Biol Chem 282:17623-31 (2007). PubMed: 17446168

      レビューと Q&A

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      1-6 of 6 Abreviews or Q&A

      Question

      Thank you very much for Proforma invoice. We will inform our customer and after then we will send an official order. But Hthe customer would like to know, if POSITIVE CINTROL is possible to buy from Abcam.

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      Answer

      Thank you for your enquiry. For ab7398 and 3145 - you can use 5-FU-resistant cancer cell lines or human breast, colorectal, gastric, head and neck carcinomas as positive controls. All the cells especially the rapidly proliferating cells express this enzyme. For details of our human tumor cell lysate, please take a look at our on-line catalogue: https://www.abcam.com/index.html?pageconfig=catalog_byproducttype&intProductTypeID=12&intProductSubTypeID=3

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      Question

      Please would you let me know the subclass of the antibody to thymidylate synthase and whether the light chain is kappa or lambda. Also, what is the sensitivity of the antibody.

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      Answer

      This is a polyclonal antibody, and so we cannot refer to subclasses. The concentration is 10mg/ml and the sensitivity can be seen from the recommended concentrations for each application.

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      Question

      Is the antibody directed to the N-terminal?

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      Answer

      This antibody was raised against the full length recombinant protien. The epitopes recognised by this antibody have not been mapped.

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      Question

      Would you please let me know what is the carrier protein for the antibody.

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      Answer

      This antibody was raised against a recombinant protein. A carrier protein was not used for immunisation.

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      Question

      Do you have an example of the results obtained for a Western Blot for this antibody. Also the quote is for 1 mg of antibody. Is this 1 mg of an IgG fraction or for purified antibody?

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      Answer

      The antibody is an IgG fraction. Unfortunatly we do not have an example to hand of the Western blot. However, the antibody works very well in Western blotting and we recommend this antibody for this application.

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      Question

      Does your antibody (THymidylate Synthase) work on paraffin?

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      Answer

      Yes, the anti-hTS does work on paraffin.

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