Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Thrombospondin antibody [A6.1]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (intestinum)
Specification
intestinum
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
Yes - PBS/TritonX
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/200
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/TritonX/goat

Secondary antibody

Name
Non-Abcam antibody was used: ABC Zytomed
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Broad spectrum streptavidin-biotin system used for detection. Able to detect primary antibodies raised in mouse, rabbit, rat and guinea pig.

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投稿 Sep 18 2012

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