Immunocytochemistry/ Immunofluorescence abreview for Anti-Thrombospondin antibody [A6.1]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary culture of astrocytes)
Specification
Primary culture of astrocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton 0.3%
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C

Other product details

Dilution
1/50
Incubation time
30 minute(s) · Temperature: 20°C · Diluent: PBS-BSA 3%

Secondary antibody

Name
Non-Abcam antibody was used: Donkey Cy3-conjugated anti mouse IgG affinity puri
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/500

Additional data

Additional Notes
PFA 4% 10 min
Permeabilisation: PBS-triton 0.3% 2 min

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投稿 Jan 14 2010

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