製品の概要

  • 製品名

    Anti-Thrombospondin antibody [A6.1]
    Thrombospondin 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [A6.1] to Thrombospondin
  • 由来種

    Mouse
  • アプリケーション

    適用あり: Flow Cyt, ICC/IF, IHC-P, IHC-Fr, IP, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Sheep, Horse, Cow, Dog, Human, Pig
  • 免疫原

    BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.

  • ポジティブ・コントロール

    • Tonsil

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

画像

  • Immunohistochemical analsysis of formalin-fixed paraffin-embedded human placenta, labeling Thrombospondin with ab1823.

  • Overlay histogram showing PC12 cells stained with ab1823 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1823, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab1823 staining Thrombospondin in a primary culture of rat astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/50: 3% BSA; PBS) for 30 minutes at 20°C. A Cy3®-conjugated-Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody.

    See Abreview

  • ICC/IF image of ab1823 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1823, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab1823 at a 1/25 dilution staining human Glioblastoma Multiform tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was stained with the antibody for 1 hour. Bound antibody was detected using biotin anti-mouse probe .

    This image is courtesy of an Abreview submitted by Francisca Wu on 14 March 2006.

    See Abreview

参考文献

This product has been referenced in:

  • Novelli EM  et al. Vascular TSP1-CD47 Signaling Promotes Sickle Cell-Associated Arterial Vasculopathy and Pulmonary Hypertension in Mice. Am J Physiol Lung Cell Mol Physiol N/A:N/A (2019). Read more (PubMed: 30892078) »
  • Fan X  et al. MicroRNA-7 Exerts Antiangiogenic Effect on Colorectal Cancer via ERK Signaling. J Surg Res 240:48-59 (2019). Read more (PubMed: 30909065) »
See all 37 Publications for this product

レビューと Q&A

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1-10 of 19 Abreviews

Application
Western blot
Sample
Rabbit Serum (Rabbit Aqueouse Humor, 20ul/well)
Gel Running Conditions
Reduced Denaturing (8-20%)
Loading amount
20 µg
Specification
Rabbit Aqueouse Humor, 20ul/well
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Xiaoqing Guo

Verified customer

投稿 Apr 30 2019

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (4-20% mini gel)
Sample
Mouse Tissue lysate - whole (Penile Tissue)
Specification
Penile Tissue
Blocking step
SuperBlock Blocking Buffer (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Miss. Gwen Lagoda

Verified customer

投稿 Nov 11 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Pig Tissue sections (liver)
Specification
liver
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Nov 29 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Rabbit Tissue sections (splen rabbit)
Specification
splen rabbit
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jun 04 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Rat Tissue sections (brain)
Specification
brain
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jun 04 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEFs)
Specification
MEFs
Fixative
Formaldehyde
Permeabilization
Yes - TBS Tw20
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

投稿 Dec 12 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (1BRG3 fibroblast)
Specification
1BRG3 fibroblast
Fixative
Formaldehyde
Permeabilization
Yes - Tween 20
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

投稿 Dec 12 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Meriones unguiculatus Tissue sections (spleen)
Specification
spleen
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Ms. Andrea Dannullis

Verified customer

投稿 Sep 25 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (intestinum)
Specification
intestinum
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
Yes - PBS/TritonX
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Sep 18 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tonsille)
Specification
tonsille
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Sep 18 2012

1-10 of 19 Abreviews

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