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Anti-Thrombomodulin 抗体 [SP309] - BSA and Azide free (ab272018)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)
  • Flow Cytometry - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)
  • Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP309] to Thrombomodulin - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-Thrombomodulin antibody [SP309] - BSA and Azide free
    Thrombomodulin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP309] to Thrombomodulin - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human mesothelioma, cervical squamous cell carcinoma, skin squamous cell carcinoma, lung squamous cell carcinoma and placenta tissues. Flow Cyt: THP-1 cells.

  • 特記事項

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab272018 is the carrier-free version of ab227701. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
IHC-P
Human
アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

 
特記事項
Flow Cyt
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

 

ターゲット情報

  • 機能

    Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

  • 組織特異性

    Endothelial cells are unique in synthesizing thrombomodulin.

  • 関連疾患

    Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
    Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.

  • 配列類似性

    Contains 1 C-type lectin domain.
    Contains 6 EGF-like domains.

  • 翻訳後修飾

    N-glycosylated.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AHUS 6 antibody
      • AHUS6 antibody
      • BDCA 3 antibody
      • BDCA3 antibody
      • CD 141 antibody
      • CD141 antibody
      • CD141 antigen antibody
      • Fetomodulin antibody
      • Thbd antibody
      • THPH12 antibody
      • THRM antibody
      • Thrombomodulin antibody
      • TM antibody
      • TRBM_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)

      Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227701).
    • Flow Cytometry - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)
      Flow Cytometry - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)

      Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling Thrombomodulin with ab227701 at 1/300 dilution (green) compared with a rabbit IgG negative control (blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227701).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)

      Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227701).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)

      Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227701).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)

      Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227701).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)

      Formalin-fixed, paraffin-embedded human placenta tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227701).
    • Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)
      Anti-Thrombomodulin antibody [SP309] - BSA and Azide free (ab272018)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272018 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272018 は論文での使用が確認できていません。

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com