Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Baboon Tissue sections (Heart)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Heart
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Other product details
Incubation time
30 minute(s) · Temperature: 24°C
Dilution
1/700
Secondary antibody
Name
Non-Abcam antibody was used: Envision+ Mouse (Dako)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Very little to zero staining seen in Baboon tissue.
Abcam response
Thank you for submitting an AbReview and your valuable feedback. We appreciate the time and effort that the reviewer has put in to testing the antibody in IHC-P on Baboon tissue, a species that has not been tested before. The results indicate that the antibody may not be suitable for this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Premier Laboratory
Verified customer
投稿 Apr 26 2016