Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (heart and lung)
Permeabilization
No
Specification
heart and lung
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
Acetone
Other product details
Incubation time
30 minute(s) · Temperature: 24°C
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit anti-Mouse and Envision+ Rabbit (Dako)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Abcam response
Thank you for submitting an Abreview and your valuable feedback. We are pleased to see that this antibody is working well for you in IHC-Fr, an application that has not been tested before. We welcome any further feedback and Abreviews from other customers who have used this antibody in IHC-Fr which will help us assess how well the antibody works in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Premier Laboratory
Verified customer
投稿 Apr 19 2016