製品の概要
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製品名Anti-Thrombomodulin antibody [PBS-01]
Thrombomodulin 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [PBS-01] to Thrombomodulin
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由来種Mouse
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アプリケーション適用あり: ELISA, WB, Flow Cyt, IHC-Pmore details
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種交差性交差種: Rabbit, Human
非交差種: Pig -
免疫原
Recombinant full length protein (Human).
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エピトープEGF domain 5 of thrombomodulin.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 6.50
Constituents: 0.292% Sodium chloride, 4% Sucrose, 0.1% Tween, 0.41% Sodium acetate -
Concentration information loading... -
精製度Protein G purified
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ポリ/モノモノクローナル
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クローン名PBS-01
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ミエローマSp2
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab6980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
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組織特異性Endothelial cells are unique in synthesizing thrombomodulin.
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関連疾患Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
配列類似性Contains 1 C-type lectin domain.
Contains 6 EGF-like domains. -
翻訳後修飾N-glycosylated.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 7056 Human
- Omim: 188040 Human
- SwissProt: P07204 Human
- Unigene: 2030 Human
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別名
- AHUS 6 antibody
- AHUS6 antibody
- BDCA 3 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (ab6980)](https://www.abcam.co.jp/ps/products/6/ab6980/Images/Thrombomodulin-Primary-antibodies-ab6980-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (ab6980)Ab6980 staining human normal lung endothelium. Staining is localized to the membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
![Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (ab6980)](https://www.abcam.co.jp/ps/products/6/ab6980/Images/Thrombomodulin-Primary-antibodies-ab6980-7.jpg)
Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (ab6980)Overlay histogram showing THP1 cells stained with ab6980 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6980, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - Anti-Thrombomodulin antibody [PBS-01] (ab6980)](https://www.abcam.co.jp/ps/products/6/ab6980/Images/Thrombomodulin-Primary-antibodies-ab6980-6.jpg)
Western blot - Anti-Thrombomodulin antibody [PBS-01] (ab6980)Image courtesy of an anonymous Abreview.Anti-Thrombomodulin antibody [PBS-01] (ab6980) at 1/100 dilution + whole cell lysate prepared from human aortic endothelial cells at 30 µg
Secondary
HRP conjugated anti-mouse IgG at 1/4000 dilution
Developed using the ECL technique.
Observed band size: 95 kDa (why is the actual band size different from the predicted?)
Exposure time: 10 minutes
36kDa band is GAPDH in the same lane.
参考文献
This product has been referenced in:
- Kim SD et al. Thrombomodulin Expression in Tissues From Dogs With Systemic Inflammatory Disease. Vet Pathol 53:797-802 (2016). Read more (PubMed: 26926084) »
- Yamaji-Kegan K et al. Hypoxia-induced mitogenic factor (FIZZ1/RELMa) induces endothelial cell apoptosis and subsequent interleukin-4-dependent pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol 306:L1090-103 (2014). Read more (PubMed: 24793164) »
レビューと Q&A
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