Anti-Thrombin 抗体 [5G9] (ab17199)

Please find the CoC forab17199 attached as requested.

If you have any further questions please do not hesitate to ask.

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Please find attached the new CoC as requested.

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A western blot which is unfortunately unavailable shows ab17199 can detect a 36kDa band and a 31 and a6kDa under reducing conditions. I have looked at western blots from other thrombinantibodies on our catalog and they also appear to detect bands around 75kDa. Its not clear if these bands are pro-thrombin or post-translationally modified thrombin.

Regarding your other question, we have no data regarding the use of ab17199 to detect recombinant thrombin.

I hope this is helpful. Please contact me again if you have any further questions.

Thank you for your enquiry.

I have checked the SwissProt entry for humanthrombin at http://www.uniprot.org/uniprot/P00734.

This link contains a thrombin sequence of 622 amino acids with predicted molecular weight of about 70kDa. This predicted mass corresponds very well to the band detected in the Human Plasma Total Protein Lysate sample shown in the western blot on the online datasheet for ab17199. Since the band runs just above 75kDa marker, at note was placed on the datasheet suggesting that gyocosylation may be responsible for this increase in apparent mass.

ab17199 may be useful for detecting recombinant thrombin, although we do not have any data regarding this.

I hope this is helpful. Please contact me again if you have any further questions.

Thank you for contacting us.
This antibody recognizes Thrombin and not ProThrombin. The higher than expected molecular weight that is seen in western blotting could be due to the glycosylation that Thrombin undergoes (based on the sequence form Swissprot http://www.uniprot.org/uniprot/P00734). This antibody binds to human thrombin and to complex of human thrombin with the thrombin inhibitor hirudin. No cross-reaction is seen with prothrombin. Cross-reactivity with thrombin from other species has not been tested.
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I have talked to the supplying lab and they have confirmed that this antibody only recognizes Thrombin and not ProThrombin. The higher than expected molecular weight that is seen in western blotting could be due to the glycosylation that Thrombin undergoes (based on the sequence form Swissprot http://www.uniprot.org/uniprot/P00734).

Please let me know if there is anything thing else I can help you with.

Thank you for your inquiry and I apologize for the delay in this reply. Yes, ab18447 is a suitable isotype control for ab17199. https://www.abcam.com/Mouse-IgG1-kappa-monoclonal-MG1-45-Isotype-control-ab18447.html I hope this information helps. Please contact us with any other questions.

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Thrombin following processing from its precursor prothrombin has a molecular weight of 36KDa (please see the wikipedia link below). However, the details that you refer to in the relevance section of this antibody; "two peptide chains of 36 and 259 amino acids" would not account for a molecular weight of 36000Da. http://en.wikipedia.org/wiki/Thrombin I would appreciate it if you could provide me with further details in order for our technical team to investigate the problems that you have been experiencing by clicking on the link below and filling in our technical questionaire. It would also be most informative if you could provide me with a representative blot and details of the batch number. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=17199&mode=questionaire I look forward to hearing from you.