Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7354] to Themis - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Human
製品名Anti-Themis antibody [EPR7354] - BSA and Azide free
Themis 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7354] to Themis - BSA and Azide free
アプリケーション適用あり: ICC, WBmore details
適用なし: Flow Cyt,IHC-P or IP
Synthetic peptide within Human Themis aa 600-700. The exact sequence is proprietary.
ab248330 is the carrier-free version of ab129174 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248330 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab248330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).|
機能Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
配列類似性Belongs to the themis family.
翻訳後修飾Phosphorylated on Tyr residues quickly after TCR stimulation.
- Information by UniProt
- C6orf190 antibody
- C6orf207 antibody
- MGC163388 antibody
All lanes : Anti-Themis antibody [EPR7354] (ab129174) at 1/10000 dilution
Lane 1 : Human thymus lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
This data was developed using ab129174, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.