Thapsigargin, Ca2+-ATPase inhibitor (ab120286)

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製品の概要

  • 製品名
    Thapsigargin, Ca2+-ATPase inhibitor
  • 製品の詳細
    Ca2+-ATPase inhibitor
  • 生理活性の詳細
    A potent, cell-permeable Ca2+-ATPase inhibitor. Releases Ca2+ by inhibiting endoplasmic reticular Ca2+-ATPase (IC50 = 4-13 nM). Both tumorogenic and apoptotic actions reported.
  • 精製度
    > 98%
  • CAS 番号
    67526-95-8
  • 構造式
    Chemical Structure

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab120286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • Functional Studies - Thapsigargin, Ca<sup>2+</sup>-ATPase inhibitor (ab120286)
    Functional Studies - Thapsigargin, Ca2+-ATPase inhibitor (ab120286)
    ab58668 staining ATF3 in serum starved A549 cells treated with thapsigargin (ab120286), by ICC/IF. Increase of ATF3 correlates with increased concentration of thapsigargin, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120286 (thapsigargin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

参考文献

This product has been referenced in:
  • Treiman M  et al. A tool coming of age: thapsigargin as an inhibitor of sarco-endoplasmic reticulum Ca(2+)-ATPases. Trends Pharmacol Sci 19:131-5 (1998). Read more (PubMed: 9612087) »
  • Inesi G & Sagara Y Thapsigargin, a high affinity and global inhibitor of intracellular Ca2+ transport ATPases. Arch Biochem Biophys 298:313-7 (1992). Read more (PubMed: 1416963) »
  • Thastrup O  et al. Thapsigargin, a novel molecular probe for studying intracellular calcium release and storage. Agents Actions 27:17-23 (1989). Read more (PubMed: 2787587) »
See all 8 Publications for this product

ab120286 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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Question

Good afternoon,

I bought one of your products (thapsigargin, ab120286) and today it has been

delivered at room temperature, but its storage, as data sheet reports, is -20°C.

I'm not sure that the shipping was appropriated and I reserve to reclaim this

productif it will have some problem.

Thank you for your attention

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Answer

Thank you for your inquiry.

I can confirm that this product is shipped at room temperature.

It has been tried and tested by us that the shipping at room temperature does not have any negative effect on the product quality.

We recommend to store it at -20C immediate after the arrival for long term storage. I would like to make you aware that the Abpromise guarantee only covers the product for six month when the product was stored as instructed on the datasheet.

If stored correctly, you will be happy to provide a free of charge replacement of credit note in the unlikely case that a product is not functioning as stated on the datasheet.

I hope I could reassure you that ab120286 will be functional. Please do not hesitate to contact me again with any further questions and concerns.

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