Thapsigargin, Ca2+-ATPase inhibitor (ab120286)

Reviews (1)Q&A (1)References (20)

製品の概要

  • 製品名

    Thapsigargin, Ca2+-ATPase inhibitor

  • 製品の詳細

    Ca2+-ATPase inhibitor

  • 生理活性の詳細

    A potent, cell-permeable Ca2+-ATPase inhibitor. Releases Ca2+ by inhibiting endoplasmic reticular Ca2+-ATPase (IC50 = 4-13 nM). Both tumorogenic and apoptotic actions reported.

  • 精製度

    > 98%

  • CAS 番号

    67526-95-8

  • 構造式

    Chemical Structure

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab120286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • Functional Studies - Thapsigargin, Ca<sup>2+</sup>-ATPase inhibitor (ab120286)
    Functional Studies - Thapsigargin, Ca2+-ATPase inhibitor (ab120286)
    ab58668 staining ATF3 in serum starved A549 cells treated with thapsigargin (ab120286), by ICC/IF. Increase of ATF3 correlates with increased concentration of thapsigargin, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120286 (thapsigargin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献

ab120286 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab120286 は 20 報の論文で使用されています。

  • Klann K  et al. Functional Translatome Proteomics Reveal Converging and Dose-Dependent Regulation by mTORC1 and eIF2a. Mol Cell 77:913-925.e4 (2020). PubMed: 31812349
  • Obiedat A  et al. The integrated stress response promotes B7H6 expression. J Mol Med (Berl) 98:135-148 (2020). PubMed: 31838577
  • Mahameed M  et al. Pharmacological induction of selective endoplasmic reticulum retention as a strategy for cancer therapy. Nat Commun 11:1304 (2020). PubMed: 32161259
  • Yang H  et al. MTORC1 coordinates the autophagy and apoptosis signaling in articular chondrocytes in osteoarthritic temporomandibular joint. Autophagy N/A:1-18 (2019). PubMed: 31007149
  • Duan J  et al. The cell-wide web coordinates cellular processes by directing site-specific Ca2+ flux across cytoplasmic nanocourses. Nat Commun 10:2299 (2019). PubMed: 31127110
View all Publications for this product

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AML12 liver cells reated with Thapsigargin

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Abreviews
abreview image
Thapsigargin treatment induced ER stress response proteins in AML12 cells after 12 hours of exposure.

Cell were splitted in 6-well plate and treated with different concentration of Thapsigargin dissolved in DMSO fo 12 hours

Western blot was performed using whole cell lysate and ER stress markers XBP,, CHOP were analysed.

Abcam user community

Verified customer

投稿 Jul 08 2020

Question

Good afternoon,

I bought one of your products (thapsigargin, ab120286) and today it has been

delivered at room temperature, but its storage, as data sheet reports, is -20°C.

I'm not sure that the shipping was appropriated and I reserve to reclaim this

productif it will have some problem.

Thank you for your attention

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Answer

Thank you for your inquiry.

I can confirm that this product is shipped at room temperature.

It has been tried and tested by us that the shipping at room temperature does not have any negative effect on the product quality.

We recommend to store it at -20C immediate after the arrival for long term storage. I would like to make you aware that the Abpromise guarantee only covers the product for six month when the product was stored as instructed on the datasheet.

If stored correctly, you will be happy to provide a free of charge replacement of credit note in the unlikely case that a product is not functioning as stated on the datasheet.

I hope I could reassure you that ab120286 will be functional. Please do not hesitate to contact me again with any further questions and concerns.

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