Anti-TGFBI 抗体 (ab99562)
Key features and details
- Goat polyclonal to TGFBI
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TGFBI antibody
TGFBI 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to TGFBI -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Dog, Pig -
免疫原
Synthetic peptide corresponding to Human TGFBI aa 119-131 (internal sequence).
Sequence:C-QLYTDRTEKLRPE
Database link: NP_000349.1 -
ポジティブ・コントロール
- WB: Human kidney tissue lysate. IHC-P: Human kidney tissue. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99562の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 75 kDa).
A 1 hour primary incubation is recommended for this product. |
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 75 kDa). A 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. -
組織特異性
Highly expressed in the corneal epithelium. -
関連疾患
Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD) [MIM:121820]; also known as Cogan corneal dystrophy or map-dot-fingerprint type corneal dystrophy. EBMD is a bilateral anterior corneal dystrophy characterized by grayish epithelial fingerprint lines, geographic map-like lines, and dots (or microcysts) on slit-lamp examination. Pathologic studies show abnormal, redundant basement membrane and intraepithelial lacunae filled with cellular debris. Although this disorder usually is not considered to be inherited, families with autosomal dominant inheritance have been identified.
Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1) [MIM:121900]; also known as corneal dystrophy granular type. Inheritance is autosomal dominant. Corneal dystrophies show progressive opacification of the cornea leading to severe visual handicap.
Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1) [MIM:122200]. Inheritance is autosomal dominant.
Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB) [MIM:602082]; also known as corneal dystrophy of Bowman layer type 2 (CDB2).
Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB) [MIM:608470]; also known as corneal dystrophy of Bowman layer type 1 (CDB1).
Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A) [MIM:608471]. CDL3A clinically resembles to lattice corneal dystrophy type 3, but differs in that its age of onset is 70 to 90 years. It has an autosomal dominant inheritance pattern.
Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD) [MIM:607541]. ACD could be considered a variant of granular dystrophy with a significant amyloidogenic tendency. Inheritance is autosomal dominant. -
配列類似性
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
翻訳後修飾
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
細胞内局在
Secreted > extracellular space > extracellular matrix. May be associated both with microfibrils and with the cell surface. - Information by UniProt
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参照データベース
- Entrez Gene: 7045 Human
- Entrez Gene: 21810 Mouse
- Entrez Gene: 100519300 Pig
- Entrez Gene: 100009230 Rabbit
- Entrez Gene: 116487 Rat
- Omim: 601692 Human
- SwissProt: P55906 Cow
- SwissProt: Q15582 Human
see all -
別名
- RGD containing collagen associated protein antibody
- AI181842 antibody
- AI747162 antibody
see all
画像
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Lanes 1-2 : Anti-TGFBI antibody (ab99562) at 0.3 µg/ml
Lane 3 : Anti-TGFBI antibody (ab99562) at 0.1 mg/ml
Lane 1 : Human Kidney in RIPA buffer
Lane 2 : Colorectal cancer in RIPA buffer
Lane 3 : Human Prostate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 75 kDa -
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ab99562 at 3.8µg/ml staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab99562 は論文での使用が確認できていません。