製品の概要

  • 製品名

    Anti-TGF beta 1 antibody [TB21]
    TGF beta 1 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [TB21] to TGF beta 1
  • 由来種

    Mouse
  • 特異性

    This antibody recognises natural and recombinant TGF beta 1. It reacts with both dimeric and monomeric natural forms under reducing and non reducing conditions. Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
  • アプリケーション

    適用あり: IHC-Fr, IHC-P, ELISA, ICC/IF, Neutralising, WBmore details
  • 種交差性

    交差種: Sheep, Human, Pig
    交差が予測される動物種: Rat
  • 免疫原

    Human TGF Beta 1 from human platelets.

  • ポジティブ・コントロール

  • 特記事項

    We have mixed feedback from customers about the Rat specificity so the Rat species has been moved to predicted as we can't guarantee it. The antibody has been successfully used with Rat samples in publication PMID 22471627 and PMID 19820199 though.

     

    This clone (TB21) demonstrates neutralising activity against TGF beta 1 in cell proliferation assays. Removal of sodium azide is recommended prior to use in functional assays.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration. PubMed: 8237223
WB 1/2000.

See Abreview. We recommend using BSA in blocking solution. Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.

ターゲット情報

  • 機能

    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • 組織特異性

    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • 関連疾患

    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • 配列類似性

    Belongs to the TGF-beta family.
  • 翻訳後修飾

    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • 細胞内局在

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース

  • 別名

    • Cartilage-inducing factor antibody
    • CED antibody
    • Differentiation inhibiting factor antibody
    • DPD1 antibody
    • LAP antibody
    • Latency-associated peptide antibody
    • Prepro transforming growth factor beta 1 antibody
    • TGF beta 1 antibody
    • TGF beta antibody
    • TGF beta 1 protein antibody
    • TGF-beta 1 protein antibody
    • TGF-beta-1 antibody
    • TGF-beta-5 antibody
    • TGF-beta1 antibody
    • TGFB antibody
    • Tgfb-1 antibody
    • tgfb1 antibody
    • TGFB1_HUMAN antibody
    • TGFbeta antibody
    • TGFbeta1 antibody
    • Transforming Growth Factor b1 antibody
    • Transforming Growth Factor beta 1 antibody
    • Transforming growth factor beta 1a antibody
    • transforming growth factor beta-1 antibody
    • transforming growth factor, beta 1 antibody
    • Transforming Growth Factor-ß1 antibody
    see all

画像

  • ab27969 staining TGF beta 1 in rat kidney tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 2% BSA for 2 hours at 25°C followed by incubation with the primary antibody, at a 1/200 dilution, for 9 hours at 4°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 594 (red) used at a 1/500 dilution.

    See Abreview

  • ab 27969 staining TGF beta 1 in human 293ft cells by Immunocytochemistry/ Immunofluorescence.
    The 293 FT cells were cultured for 3 days then fixed with 3.7% formaldehyde for 10 minutes and blocked with 5% BSA in PBS for 1 hour at +4°C. The cells were incubated with the primary antibody at 1/200 dilution overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 555 Goat anti-Rabbit IgG used at a dilution of 1/200.
  • ab27969 staining TGF beta 1 in Human ureter tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/150) for 16 hours at 4°C. A Cy2®-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:

  • Gu TT  et al. Pterostilbene alleviates fructose-induced renal fibrosis by suppressing TGF-ß1/TGF-ß type I receptor/Smads signaling in proximal tubular epithelial cells. Eur J Pharmacol 842:70-78 (2019). Read more (PubMed: 30336139) »
  • Wang N  et al. Carcinoembryonic antigen cell adhesion molecule 1 inhibits the antitumor effect of neutrophils in tongue squamous cell carcinoma. Cancer Sci 110:519-529 (2019). Read more (PubMed: 30565803) »
See all 40 Publications for this product

レビューと Q&A

1-10 of 10 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human skin)
Specification
Human skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jul 31 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Ureter)
Specification
Ureter
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Mar 14 2013

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Ureter)
Specification
Ureter
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Ms. Beate Thode

Verified customer

投稿 Mar 14 2013

Application
Western blot
Sample
Human Tissue lysate - whole (Human Penile Tissue)
Loading amount
60 µg
Specification
Human Penile Tissue
Gel Running Conditions
Reduced Denaturing (4-20% mini gel)
Blocking step
SuperBlock Blocking Buffer (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Miss. Gwen Lagoda

Verified customer

投稿 Jul 26 2012

Answer

Thank you for your enquiry and for your patience. It has taken some time to confirm this information for you with our source. I can confirm that this antibody has been used at 1:1000 successfully. I have added this to the datasheet. However, other researchers have needed to use it at 1:10. The dilutions provided on the online datasheet are a guideline only. Individual customers will usually need to optimize the dilution for their individual experiments. I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for your email! The best way to figure out dilutions and number of tests is to look at the datasheet. In most cases we will have a recommended dilution for each application, and will also include the concentration of the product along with the amount of product in the vial. Using these pieces of information, you can get the number of experiments that you will be able to do with that product. In order to give you the number of tests, I will need to know what application you are looking to use the antibodies in. The calculator at the following link may help you: https://www.msu.edu/~nixonjos/more/dilution.html?stock=1&needed=0.0001&volume=1&total=0.1 To get the number of tests, you can divide the final result ("total stock solution to use") by the amount included in the tube. I hope this helps. If you have any further questions, please do not hesitat

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Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (kidney)
Specification
kidney
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Sep 24 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (293FT cell line)
Specification
293FT cell line
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

投稿 Jan 14 2010

Application
Western blot
Sample
Human Tissue lysate - whole (heart)
Loading amount
50 µg
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

投稿 Nov 01 2006

Answer

Thank you for your enquiry. Following an investigation it appears that ab27969 is unconjugated and it is not guaranteed for use in Flow Cytometry. Unfortunately we don't have an antibody to TGFb that is conjugated and approved for flow cytometry. I'm sorry for this mistake. Please let me know if I can be of further assistance,

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