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RabMAb

Anti-TGF beta 1 抗体 [EPR18163] (ab179695)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18163] to TGF beta 1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-TGF beta 1 antibody [EPR18163]
    TGF beta 1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR18163] to TGF beta 1

  • 由来種

    Rabbit

  • 特異性

    ab179695 recognizes the mature and cleaved forms of TGF-beta 1.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment within Mouse TGF beta 1 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P04202

  • ポジティブ･コントロール

    • WB: L-929, RAW 264.7, HeLa, A549 and K562 whole cell lysates; Mouse serum; Mouse spleen lysate; Rat kidney and spleen lysates; Rat serum; Human kidney and spleen lysates; Recombinant protein fragment human TGF beta 1.

  • 特記事項

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab179695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 50, 12.5 kDa (predicted molecular weight: 44 kDa).

ターゲット情報

  • 機能

    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

  • 組織特異性

    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.

  • 関連疾患

    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.

  • 配列類似性

    Belongs to the TGF-beta family.

  • 翻訳後修飾

    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Cartilage-inducing factor antibody
    • CED antibody
    • Differentiation inhibiting factor antibody
    • DPD1 antibody
    • LAP antibody
    • Latency-associated peptide antibody
    • Prepro transforming growth factor beta 1 antibody
    • TGF beta 1 antibody
    • TGF beta antibody
    • TGF beta 1 protein antibody
    • TGF-beta 1 protein antibody
    • TGF-beta-1 antibody
    • TGF-beta-5 antibody
    • TGF-beta1 antibody
    • TGFB antibody
    • Tgfb-1 antibody
    • tgfb1 antibody
    • TGFB1_HUMAN antibody
    • TGFbeta antibody
    • TGFbeta1 antibody
    • Transforming Growth Factor b1 antibody
    • Transforming Growth Factor beta 1 antibody
    • Transforming growth factor beta 1a antibody
    • transforming growth factor beta-1 antibody
    • transforming growth factor, beta 1 antibody
    • Transforming Growth Factor-ß1 antibody
    see all

画像

  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Wild-type HeLa cell lysate
    Lane 4 : TGF beta 1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 44 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab179695 observed at 50 kDa. Red - loading control, ab8245 observed at 37 kDa.  

     ab179695 was shown to react with TGF beta 1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255439 (knockout cell lysate ab263799) was used. Wild-type and TGF beta 1 knockout samples were subjected to SDS-PAGE. ab179695 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/5000 dilution

    Lane 1 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2-4:30 seconds.

     

  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Lane 1 : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/5000 dilution
    Lanes 2-5 : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution

    Lane 1 : Mouse serum
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : Rat serum

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 12.5,50 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2/3/5: 3minutes; Lane 4: 30 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID:18390240; PMID:12717387; PMID:2139036).

  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution

    Lane 1 : Human kidney lysate
    Lane 2 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 12.5,50 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:18390240; PMID:12717387; PMID:2139036).

  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution

    Lane 1 : Recombinant protein fragment human TGF beta 1
    Lane 2 : Recombinant protein fragment human TGF beta 2
    Lane 3 : Recombinant protein fragment human TGF beta 3

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2/3: 3 minutes.

    Recombinant protein fragment human TGF beta 1 contains aa279-390 with a GST-tag. Recombinant protein fragment human TGF beta 2 contains aa303-414 with a GST-tag Recombinant protein fragment human TGF beta 3 contains aa301-412 with a GST-tag. These three fragment recombinant proteins were made in-house. 

参考文献 (19)

ab179695 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab179695 は 19 報の論文で使用されています。

  • Kuma A  et al. Inhibition of urea transporter ameliorates uremic cardiomyopathy in chronic kidney disease. FASEB J 34:8296-8309 (2020). PubMed: 32367640
  • Rianto F  et al. UT-A1/A3 knockout mice show reduced fibrosis following unilateral ureteral obstruction. Am J Physiol Renal Physiol 318:F1160-F1166 (2020). PubMed: 32174141
  • Xue C  et al. ß-Elemene suppresses the proliferation of human airway granulation fibroblasts via attenuation of TGF-ß/Smad signaling pathway. J Cell Biochem 120:16553-16566 (2019). PubMed: 31104326
  • Jiang ZJ  et al. Galectin-1 gene silencing inhibits the activation and proliferation but induces the apoptosis of hepatic stellate cells from mice with liver fibrosis. Int J Mol Med 43:103-116 (2019). PubMed: 30365068
  • MacFarlane EG  et al. Lineage-specific events underlie aortic root aneurysm pathogenesis in Loeys-Dietz syndrome. J Clin Invest 129:659-675 (2019). PubMed: 30614814
View all Publications for this product

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Western blot abreview for Anti-TGF beta 1 antibody [EPR18163]

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Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Sep 06 2018

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