製品の概要

  • 製品名
    Anti-TGF beta 1 antibody [2E6]
    TGF beta 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2E6] to TGF beta 1
  • 由来種
    Mouse
  • 特異性
    Western blotting demonstrated that this antibody reacts with the dimeric (25 kDa) and monomeric (12.5 kDa.) forms of TGF-beta under both non-reducing and reducing conditions respectively. TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. This antibody recognizes both human platelet-derived and recombinant TGF-beta in ELISA.
  • アプリケーション
    適用あり: Neutralising, WB, ELISAmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human TGF beta 1. TGF beta 1 from human platelets.

  • ポジティブ・コントロール
    • WB: recombinant protein
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Neutralising Use at an assay dependent concentration. PubMed: 17574548
WB Use a concentration of 1 - 10 µg/ml.

Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.This antibody, used at this concentration will allow visualization of 100 ng/lane of TFG-beta.

ELISA Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
    • 組織特異性
      Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
    • 関連疾患
      Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
    • 配列類似性
      Belongs to the TGF-beta family.
    • 翻訳後修飾
      Glycosylated.
      The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
    • 細胞内局在
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cartilage-inducing factor antibody
      • CED antibody
      • Differentiation inhibiting factor antibody
      • DPD1 antibody
      • LAP antibody
      • Latency-associated peptide antibody
      • Prepro transforming growth factor beta 1 antibody
      • TGF beta 1 antibody
      • TGF beta antibody
      • TGF beta 1 protein antibody
      • TGF-beta 1 protein antibody
      • TGF-beta-1 antibody
      • TGF-beta-5 antibody
      • TGF-beta1 antibody
      • TGFB antibody
      • Tgfb-1 antibody
      • tgfb1 antibody
      • TGFB1_HUMAN antibody
      • TGFbeta antibody
      • TGFbeta1 antibody
      • Transforming Growth Factor b1 antibody
      • Transforming Growth Factor beta 1 antibody
      • Transforming growth factor beta 1a antibody
      • transforming growth factor beta-1 antibody
      • transforming growth factor, beta 1 antibody
      • Transforming Growth Factor-ß1 antibody
      see all

    プロトコール

    参考文献

    This product has been referenced in:
    • Rocks N  et al. ADAMTS-1 metalloproteinase promotes tumor development through the induction of a stromal reaction in vivo. Cancer Res 68:9541-50 (2008). WB ; Human . Read more (PubMed: 19010931) »
    • Schmid SA  et al. Lactate adversely affects the in vitro formation of endothelial cell tubular structures through the action of TGF-beta1. Exp Cell Res 313:2531-49 (2007). Read more (PubMed: 17574548) »
    See all 3 Publications for this product

    レビューと Q&A

    1-10 of 16 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Guinea pig Tissue lysate - whole (Brain)
    Loading amount
    30 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Yvonne Sondy

    Verified customer

    投稿 May 21 2012

    Answer

    Thank you for your reply.

    I am sorry about the misunderstanding about the testing discount codes and I will make an exception this time due to the misunderstanding, however in future we do ask that you contact us prior to purchasing an antibody that you would like a testing discount for.

    Please let me know if there is anything else I can help you with.

    DISCOUNT CODE: *******
    Expiration date: **********

    I am very pleased to hear you would like to accept our offer and test ab647 in guinea pig tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forguinea pig tissueand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you for contacting Abcam.

    Normally we issue you the testing discount code before you purchase the antibody. From the order information you provided, we sent you ab647 as a free of charge replacement for ab9758. When my colleague sent you the replacement, did they also mention that they would send you a testing discount code? Or are enquiring as to whether you can receive a testing discount for testing ab647 in guinea pig samples, after you have already received.

    Thank you for clarifying this for me, I just want to make sure that I understand the situation.

    I look forward to your reply.

    Read More

    Answer

    Thank you for your enquiry.
    I can confirm that ab647 (anti-TGF beta 1 antibody) has been tested and characterized for detecting sheep and human TGF beta 1.
    ab50036 is a recombinant human, (mature) protein expressed in mammalian A293 cells. This recombinant protein is a 25.0 kDa protein with each subunit containing (C terminal) 112 amino acid residues. It has been tested in functional assay and it can also be used as positive control in Western blot application.
    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Answer

    Please find attached the certificates of compliance for ab647 and ab27969. If there is anything else I can help you with, please let me know.

    Read More

    Answer

    Please find below the information I was able to find: ab647 TGF beta antibody [2E6] had been demonstrated to have neutralization activity in the original paper: Schmid SA et al. Lactate adversely affects the in vitro formation of endothelial cell tubular structures through the action of TGF-beta1. Exp Cell Res 313:2531-49 (2007). PubMed: 17574548 ab1279 TGF beta antibody [TB21] had been demonstrated to have neutralization activityin the original paper: Shi Wei-Kang, et al, Biological characterization of a monoclonal antibody TB21 against human transforming growth factor-beta 1; Acta Biologica Experimentals Sinica, Vol 26(2), p141-150, 1993 The data of neutralization activity of ab30838 TGF beta antibody is not available unfortunately. I am unsure as to whether you wanted a TNF alpha antibody too so enclose the information just in case: ab34839 TNF alpha antibody - Azide free : The only information we have available regarding the use of this product in neutralizations is as follows: This antibody is useful for neutralization of human and primate TNFalpha activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. I hope this information will help you find the most suitable antibody for your experiments. Good luck with those,

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - other (Fibroblast)
    Loading amount
    30 µg
    Specification
    Fibroblast
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

    Abcam user community

    Verified customer

    投稿 Nov 21 2006

    Answer

    Thank you very much for providing me with the detailed protocol, it is very evident that the antibody does not work in IHC-P and we have immediately amended our datasheet to reflect this. I would like to send you a free vial of a different antibody, ab1279, which is also a monoclonal recognising TGF beta and detects TGF beta in ovine ovarian tissue and breast cancer sections. Can you please give me the PO number and/or Abcam order number and the name of the person who placed the order and I will arrange ASAP for a vial to be sent to you. I look forward to hearing from you and wish you all the best with your thesis write up,

    Read More

    Answer

    Thank you for the details regarding your protocol. I have a few more questions which would really help me get to the bottom of your problem: -what wax removal steps do you use? -the H2O2 step: what is the buffer? -the methanol step: is it ice cold? -which of these steps lasts 20min? Is each step lasting 20min? Have you tried without the methanol step? -the citrate buffer used to do antigen retrieval: what is the pH, concentration and how long do you leave it on for? -can you tell me the primary antibody concentration, buffer, incubation time and temperature -same for the secondary Could you provide an image to show me the staining you get? I look forward to hearing from you, Thank you for your patience,

    Read More

    Answer

    Thank you for your patience. The cap on the antibody vial should not have been loose and I do apologize for that. I'm sending you a replacement vial free of charge; for your record it is order# 43618 and you will receive the antibody this week. Please let me know if you experience any problems with the replacement.

    Read More

    1-10 of 16 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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