リコンビナントRabMAb

Anti-TFEB 抗体 [EPR22940-151] (ab270604)

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Western blot - Anti-TFEB antibody [EPR22940-151] (ab270604)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFEB antibody [EPR22940-151] (ab270604)
  • Immunocytochemistry/ Immunofluorescence - Anti-TFEB antibody [EPR22940-151] (ab270604)
  • Immunoprecipitation - Anti-TFEB antibody [EPR22940-151] (ab270604)
  • Flow Cytometry - Anti-TFEB antibody [EPR22940-151] (ab270604)
  • Flow Cytometry - Anti-TFEB antibody [EPR22940-151] (ab270604)
  • Immunocytochemistry/ Immunofluorescence - Anti-TFEB antibody [EPR22940-151] (ab270604)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFEB antibody [EPR22940-151] (ab270604)
  • Anti-TFEB antibody [EPR22940-151] (ab270604)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22940-151] to TFEB
  • Suitable for: ICC/IF, IP, Flow Cyt, IHC-P, WB
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-TFEB antibody [EPR22940-151]
    TFEB 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22940-151] to TFEB

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: IM-9 and Raji whole cell lysate. IHC-P: Human lung cancer and colon tissue. ICC/IF: IM-9 and Raji cells. Flow Cyt: IM-9 and Raji cells. IP: Raji whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Human
アプリケーション Abreviews 特記事項
ICC/IF
1/50.
IP
1/30.
Flow Cyt
1/500.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 53 kDa).
特記事項
ICC/IF
1/50.
IP
1/30.
Flow Cyt
1/500.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 53 kDa).

ターゲット情報

  • 機能

    Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.

  • 配列類似性

    Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.

  • ドメイン

    The leucin zipper region is essential for homo- or heterodimerization and high-affinity DNA binding. DNA binding is mediated by the basic region.

  • 翻訳後修飾

    Sumoylated; does not affect dimerization with MITF.

  • 細胞内局在

    Cytoplasm. Nucleus. Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Alpha TFEB antibody
      • AlphaTFEB antibody
      • bHLHe35 antibody
      • Class E basic helix-loop-helix protein 35 antibody
      • T cell transcription factor EB antibody
      • TCFEB antibody
      • TFEB antibody
      • TFEB_HUMAN antibody
      • Transcription factor EB antibody
      see all

    画像

    • Western blot - Anti-TFEB antibody [EPR22940-151] (ab270604)
      Western blot - Anti-TFEB antibody [EPR22940-151] (ab270604)
      All lanes : Anti-TFEB antibody [EPR22940-151] (ab270604) at 1/1000 dilution

      Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
      Lane 2 : IM-9 (human multiple myeloma B Lymphoblast), whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 53 kDa
      Observed band size: 66 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID:30519051).

      Exposure time: 26 secs.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFEB antibody [EPR22940-151] (ab270604)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFEB antibody [EPR22940-151] (ab270604)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TFEB with ab270604 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human colon tissue is observed (PMID:30519051). The section was incubated with ab270604 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-TFEB antibody [EPR22940-151] (ab270604)
      Immunocytochemistry/ Immunofluorescence - Anti-TFEB antibody [EPR22940-151] (ab270604)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji cells labeling TFEB with ab270604 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Raji cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Immunoprecipitation - Anti-TFEB antibody [EPR22940-151] (ab270604)
      Immunoprecipitation - Anti-TFEB antibody [EPR22940-151] (ab270604)

      TFEB was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate with ab270604 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270604 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

      Lane 1: Raji whole cell lysate.

      Lane 2: ab270604 IP in Raji whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270604 in Raji whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 secs.

       

    • Flow Cytometry - Anti-TFEB antibody [EPR22940-151] (ab270604)
      Flow Cytometry - Anti-TFEB antibody [EPR22940-151] (ab270604)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized IM-9 (Human multiple myeloma B Lymphoblast) cells labeling TFEB with ab270604 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    • Flow Cytometry - Anti-TFEB antibody [EPR22940-151] (ab270604)
      Flow Cytometry - Anti-TFEB antibody [EPR22940-151] (ab270604)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling TFEB with ab270604 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-TFEB antibody [EPR22940-151] (ab270604)
      Immunocytochemistry/ Immunofluorescence - Anti-TFEB antibody [EPR22940-151] (ab270604)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized IM-9 cells labeling TFEB with ab270604 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in IM-9 cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFEB antibody [EPR22940-151] (ab270604)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFEB antibody [EPR22940-151] (ab270604)

      Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling TFEB with ab270604 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human lung cancer tissue is observed (PMID:26264650). The section was incubated with ab270604 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-TFEB antibody [EPR22940-151] (ab270604)
      Anti-TFEB antibody [EPR22940-151] (ab270604)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab270604 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270604 は論文での使用が確認できていません。

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