Anti-TFEB 抗体 (ab2636)
Key features and details
- Goat polyclonal to TFEB
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TFEB antibody
TFEB 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to TFEB -
由来種
Goat -
特異性
This antibody is raised against TCFEB. However a customer reports that this antibody cross reacts with mouse TFE3 and MITF (other members of transcription factor family Mi/Tfe) in over-expressing cell lines. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig, Chinese hamster
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免疫原
Synthetic peptide corresponding to Human TFEB aa 2-14 (N terminal).
Sequence:ASRIGLRMQLMRE
Database link: P19484 -
ポジティブ･コントロール
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特記事項
Based on sequence homology, TFEB isoforms 1 and 2 (NP_009093.1 and NP_001161299.2) with MW of 52.9 and 54.2 kDa respectively can be detected by this antibody
Principal Names - TFEB; transcription factor EB; TCFEB; T-cell transcription factor EB Official Gene Symbol - TFEB GenBank Accession Number – NP_009093 LocusLink ID - 7942 (human); 21425 (mouse)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2636の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
Use a concentration of 0.3 - 1 µg/ml.
Primary incubation 1 hour at room temperature. Blocking in milk. Detects band(s) of approximately 53 kDa. Predicted molecular weights of TFEB isoforms 1 and 2 are 52.9 and 54.2 kDa respectively.
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| 特記事項 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Primary incubation 1 hour at room temperature. Blocking in milk. Detects band(s) of approximately 53 kDa. Predicted molecular weights of TFEB isoforms 1 and 2 are 52.9 and 54.2 kDa respectively.
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ターゲット情報
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機能
Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. -
配列類似性
Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
ドメイン
The leucin zipper region is essential for homo- or heterodimerization and high-affinity DNA binding. DNA binding is mediated by the basic region. -
翻訳後修飾
Sumoylated; does not affect dimerization with MITF. -
細胞内局在
Cytoplasm. Nucleus. Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 510032 Cow
- Entrez Gene: 609046 Dog
- Entrez Gene: 7942 Human
- Entrez Gene: 21425 Mouse
- Entrez Gene: 100157940 Pig
- Entrez Gene: 316214 Rat
- Omim: 600744 Human
- SwissProt: P19484 Human
see all -
別名
- Alpha TFEB antibody
- AlphaTFEB antibody
- bHLHe35 antibody
see all
画像
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Western blot - Anti-TFEB antibody (ab2636)Lane 1 : Anti-TFEB antibody (ab2636) at 0.5 µg/ml
Lane 2 : Anti-TFEB antibody (ab2636) at 0.3 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysatePrimary incubation was 1 hour. Detected by chemiluminescence.
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Western blot - Anti-TFEB antibody (ab2636)Anti-TFEB antibody (ab2636) at 1 µg/ml + Human thymus lysate in RIPA buffer at 35 µg
Observed band size: 60 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (47)
ab2636 は 47 報の論文で使用されています。
- Jiang Y et al. Basic fibroblast growth factor accelerates myelin debris clearance through activating autophagy to facilitate early peripheral nerve regeneration. J Cell Mol Med 25:2596-2608 (2021). PubMed: 33512767
- Tan A et al. TFEB regulates pluripotency transcriptional network in mouse embryonic stem cells independent of autophagy-lysosomal biogenesis. Cell Death Dis 12:343 (2021). PubMed: 33795648
- Li R et al. RIP3 impedes transcription factor EB to suppress autophagic degradation in septic acute kidney injury. Cell Death Dis 12:593 (2021). PubMed: 34103472
- Rao S et al. Salmonella and S. aureus Escape From the Clearance of Macrophages via Controlling TFEB. Front Microbiol 11:573844 (2020). PubMed: 33324360
- Mingione A et al. Inhibition of Sphingolipid Synthesis as a Phenotype-Modifying Therapy in Cystic Fibrosis. Cell Physiol Biochem 54:110-125 (2020). PubMed: 31999897