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  1. Link

    tfeb-antibody-ab2636.pdf

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Anti-TFEB 抗体 (ab2636)

  • Datasheet
  • SDS
Reviews (4)Q&A (1)References (47)

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Abpromise

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Western blot - Anti-TFEB antibody (ab2636)
  • Western blot - Anti-TFEB antibody (ab2636)

Key features and details

  • Goat polyclonal to TFEB
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-TFEB antibody [EPR22940-151] (ab270604)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-TFEB antibody
    TFEB 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to TFEB
  • 由来種

    Goat
  • 特異性

    This antibody is raised against TCFEB. However a customer reports that this antibody cross reacts with mouse TFE3 and MITF (other members of transcription factor family Mi/Tfe) in over-expressing cell lines.
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig, Chinese hamster
  • 免疫原

    Synthetic peptide corresponding to Human TFEB aa 2-14 (N terminal).
    Sequence:

    ASRIGLRMQLMRE


    Database link: P19484
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • ウェスタン・ブロット用ポジティブコントロールRecombinant Human TFEB protein
  • 特記事項

    Based on sequence homology, TFEB isoforms 1 and 2 (NP_009093.1 and NP_001161299.2) with MW of 52.9 and 54.2 kDa respectively can be detected by this antibody

    Principal Names - TFEB; transcription factor EB; TCFEB; T-cell transcription factor EB Official Gene Symbol - TFEB GenBank Accession Number – NP_009093 LocusLink ID - 7942 (human); 21425 (mouse)  

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

関連製品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • BALB/3T3 whole cell lysate (ab7901)
  • Recombinant Protein

    • Recombinant Human TFEB protein (ab152831)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2636の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (4)
Use a concentration of 0.3 - 1 µg/ml.

Primary incubation 1 hour at room temperature. Blocking in milk. 

Detects band(s) of approximately 53 kDa. Predicted molecular weights of TFEB isoforms 1 and 2 are 52.9 and 54.2 kDa respectively. 

 

特記事項
WB
Use a concentration of 0.3 - 1 µg/ml.

Primary incubation 1 hour at room temperature. Blocking in milk. 

Detects band(s) of approximately 53 kDa. Predicted molecular weights of TFEB isoforms 1 and 2 are 52.9 and 54.2 kDa respectively. 

 

ターゲット情報

  • 機能

    Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.
  • 配列類似性

    Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • ドメイン

    The leucin zipper region is essential for homo- or heterodimerization and high-affinity DNA binding. DNA binding is mediated by the basic region.
  • 翻訳後修飾

    Sumoylated; does not affect dimerization with MITF.
  • 細胞内局在

    Cytoplasm. Nucleus. Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus.
  • Target information above from: UniProt accession P19484 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 510032 Cow
    • Entrez Gene: 609046 Dog
    • Entrez Gene: 7942 Human
    • Entrez Gene: 21425 Mouse
    • Entrez Gene: 100157940 Pig
    • Entrez Gene: 316214 Rat
    • Omim: 600744 Human
    • SwissProt: P19484 Human
    • SwissProt: Q9R210 Mouse
    • Unigene: 485360 Human
    • Unigene: 715310 Human
    • Unigene: 2305 Mouse
    see all
  • 別名

    • Alpha TFEB antibody
    • AlphaTFEB antibody
    • bHLHe35 antibody
    • Class E basic helix-loop-helix protein 35 antibody
    • T cell transcription factor EB antibody
    • TCFEB antibody
    • TFEB antibody
    • TFEB_HUMAN antibody
    • Transcription factor EB antibody
    see all

画像

  • Western blot - Anti-TFEB antibody (ab2636)
    Western blot - Anti-TFEB antibody (ab2636)
    Lane 1 : Anti-TFEB antibody (ab2636) at 0.5 µg/ml
    Lane 2 : Anti-TFEB antibody (ab2636) at 0.3 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate


    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Western blot - Anti-TFEB antibody (ab2636)
    Western blot - Anti-TFEB antibody (ab2636)
    Anti-TFEB antibody (ab2636) at 1 µg/ml + Human thymus lysate in RIPA buffer at 35 µg

    Observed band size: 60 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

プロトコール

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (47)

ab2636 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2636 は 47 報の論文で使用されています。

  • Jiang Y  et al. Basic fibroblast growth factor accelerates myelin debris clearance through activating autophagy to facilitate early peripheral nerve regeneration. J Cell Mol Med 25:2596-2608 (2021). PubMed: 33512767
  • Tan A  et al. TFEB regulates pluripotency transcriptional network in mouse embryonic stem cells independent of autophagy-lysosomal biogenesis. Cell Death Dis 12:343 (2021). PubMed: 33795648
  • Li R  et al. RIP3 impedes transcription factor EB to suppress autophagic degradation in septic acute kidney injury. Cell Death Dis 12:593 (2021). PubMed: 34103472
  • Rao S  et al. Salmonella and S. aureus Escape From the Clearance of Macrophages via Controlling TFEB. Front Microbiol 11:573844 (2020). PubMed: 33324360
  • Mingione A  et al. Inhibition of Sphingolipid Synthesis as a Phenotype-Modifying Therapy in Cystic Fibrosis. Cell Physiol Biochem 54:110-125 (2020). PubMed: 31999897
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-TFEB antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (MEFs)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Read More

Abcam user community

Verified customer

投稿 Jul 04 2018

Western blot abreview for Anti-TFEB antibody - ChIP Grade

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex® 10% gel)
Loading amount
30 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

DR. Insu Kwon

Verified customer

投稿 Dec 10 2015

Western blot abreview for Anti-TFEB antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
35 µg
Specification
Brain
Blocking step
Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Read More

Abcam user community

Verified customer

投稿 Jan 22 2015

Western blot abreview for Anti-TFEB antibody - ChIP Grade

Average
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (breast cancer cell lines)
Loading amount
40 µg
Specification
breast cancer cell lines
Gel Running Conditions
Reduced Denaturing (8 % acrylamide gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 01 2010

Question

Hello, I recently purchased Anti-TFEB antibody - ChIP Grade (ab2636). Since it says that it is "ChIP grade,' my lab is planning on doing a few experiments involving ChIP, but before we do so we were wondering what genes/promotors abcam has used to verify that this antibody is suitable for ChIP? As much detailed information including controls, cell/tissue types probed, reaction conditions and buffers, etc. would be extremely helpful.

Read More

Abcam community

Verified customer

Asked on May 10 2012

Answer

Thank you for your inquiry.

We do not batch test this antibody for ChIP in-house, but this antibody has been successfully used in ChIP as described in the following papers:

Esumi N et al. VMD2 promoter requires two proximal E-box sites for its activity in vivo and is regulated by the MITF-TFE family. J Biol Chem 282:1838-50 (2007). PMID: 17085443.

Huan C et al. Renal Carcinoma-associated Transcription Factors TFE3 and TFEB Are Leukemia Inhibitory Factor-responsive Transcription Activators of E-cadherin. J Biol Chem 280:30225-35 (2005). PubMed: 15994295

We would guarantee this antibody to work in ChIP in accordance with our Abpromise.

I hope this information helps. Please contact us with any other questions.

Read More

Abcam Scientific Support

Answered on May 10 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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