Question (75171) | Tetrodotoxin, Na+ channel blocker (ab120054)

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Question

What molarity citrate buffer should be used for reconstitution?

Answer

The specific molarity of the citrate buffer used to solubilize TTX (ab120054) is not as significant as the pH of 4.8. You can essentially use the molarity of citrate buffer that will be applicable with your specific study. As a general guideline for a range that can be used you can use 20 mM Citrate buffer. I also found the following paper which may be helpful, which used 1.5 mM citrate buffer, pH 4.8 to dissolve TTX to a concentration of 300 or 500 uM.

http://www.jneurosci.org/content/17/11/4359.full.pdf

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