Anti-Tetraspanin-33 抗体 [EPR23121-146] - BSA and Azide free (ab272461)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23121-146] to Tetraspanin-33 - BSA and Azide free
- Suitable for: Dot blot, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Tetraspanin-33 antibody [EPR23121-146] - BSA and Azide free
Tetraspanin-33 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23121-146] to Tetraspanin-33 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: Dot blot, Flow Cytmore details
適用なし: ICC/IF,IHC-P,IP or WB -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow cyt: Human PBMCs.
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特記事項
ab272461 is the carrier-free version of ab238140.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23121-146 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272461の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Dot blot |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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特記事項 |
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Dot blot
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays an important role in normal erythropoiesis. It has a role in the differentiation of erythroid progenitors. -
組織特異性
Predominantly expressed in erythroblasts. -
配列類似性
Belongs to the tetraspanin (TM4SF) family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 340348 Human
- Omim: 610120 Human
- SwissProt: Q86UF1 Human
- Unigene: 27267 Human
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別名
- 1300010A20Rik antibody
- AI035228 antibody
- hPen antibody
see all
画像
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Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) treated with 1ug/ml human CD40 ligand and 100U/ml human IL-4 for 72h labeling Tetraspanin-33 with ab238140 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG Isotype control (ab172730) (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab238140 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238140).
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Dot blot analysis of Tetraspanin-33 labeled with ab238140 at 1/1000 dilution.
Lane 1: Recombinant tetraspanin-33 fragment containing the sequence at the immunogen region.
Lane 2: BSA.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238140).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab272461 は論文での使用が確認できていません。