Anti-Tetraspanin-33 抗体 [EPR23121-146] - BSA and Azide free (ab272461)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23121-146] to Tetraspanin-33 - BSA and Azide free
- Suitable for: Dot blot, Flow Cyt
- Reacts with: Human
製品の概要
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製品名
Anti-Tetraspanin-33 antibody [EPR23121-146] - BSA and Azide free
Tetraspanin-33 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23121-146] to Tetraspanin-33 - BSA and Azide free -
由来種
Rabbit -
Tested Applications & Species
Application Species Dot Recombinant fragmentFlow Cyt Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow cyt: Human PBMCs.
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特記事項
ab272461 is the carrier-free version of ab238140. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23121-146 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
アプリケーション | Species |
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Dot |
Recombinant fragment
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Flow Cyt |
Human
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アプリケーション | Abreviews | 特記事項 |
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Dot blot |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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特記事項 |
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Dot blot
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays an important role in normal erythropoiesis. It has a role in the differentiation of erythroid progenitors. -
組織特異性
Predominantly expressed in erythroblasts. -
配列類似性
Belongs to the tetraspanin (TM4SF) family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 340348 Human
- Omim: 610120 Human
- SwissProt: Q86UF1 Human
- Unigene: 27267 Human
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別名
- 1300010A20Rik antibody
- AI035228 antibody
- hPen antibody
see all
画像
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Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) treated with 1ug/ml human CD40 ligand and 100U/ml human IL-4 for 72h labeling Tetraspanin-33 with ab238140 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG Isotype control (ab172730) (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab238140 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238140).
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Dot blot analysis of Tetraspanin-33 labeled with ab238140 at 1/1000 dilution.
Lane 1: Recombinant tetraspanin-33 fragment containing the sequence at the immunogen region.
Lane 2: BSA.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238140).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab272461 は論文での使用が確認できていません。