Application
Western blot
Sample
Rat Cell lysate - whole cell (HEK and NRK)
Gel Running Conditions
Reduced Denaturing (6)
Loading amount
20 µg
Specification
HEK and NRK
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TTBS
Concentration
1 µg/ml
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Rabbit IgG HRP Linked Whole Ab (from Donkey)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 second(s)
Bands
Specific: 250 kDa Non-specific: 75 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 28 2017