製品の概要
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製品名Anti-Tenascin C antibody [T2H5]
Tenascin C 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [T2H5] to Tenascin C
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由来種Mouse
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アプリケーション適用あり: IHC-P, IP, WB, ICC/IFmore details
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種交差性交差種: Human
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免疫原
Full length native protein (purified from a homogenate of a mammary tumour specimen) (Human).
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ポジティブ･コントロール
- Human tonsil tissue
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特記事項
Tenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度>95% by SDS-PAGE
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特記事項（精製）Purified from hybridoma culture supernatant by protein A-affinity chromatography.
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一次抗体 備考Tenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted.
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ポリ/モノモノクローナル
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クローン名T2H5
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ミエローマunknown
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アイソタイプIgG1
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軽鎖の種類unknown
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研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab3970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 240 kDa. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 20435685 |
ターゲット情報
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機能Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
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配列類似性Belongs to the tenascin family.
Contains 15 EGF-like domains.
Contains 1 fibrinogen C-terminal domain.
Contains 15 fibronectin type-III domains. -
細胞内局在Secreted > extracellular space > extracellular matrix.
- Information by UniProt
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参照データベース
- Entrez Gene: 3371 Human
- Omim: 187380 Human
- SwissProt: P24821 Human
- Unigene: 143250 Human
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別名
- 150 225 antibody
- Cytotactin antibody
- Glioma associated extracellular matrix antigen antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [T2H5] (ab3970)](https://www.abcam.co.jp/ps/products/3/ab3970/Images/ab3970_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [T2H5] (ab3970)ab3970 staining paraffin embedded tissue sections.
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![Western blot - Anti-Tenascin C antibody [T2H5] (ab3970)](https://www.abcam.co.jp/ps/products/3/ab3970/Images/Tenascin-C-Primary-antibodies-ab3970-2.jpg)
Western blot - Anti-Tenascin C antibody [T2H5] (ab3970)Anti-Tenascin C antibody [T2H5] (ab3970) at 1 µg/ml + U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 265 kDa (why is the actual band size different from the predicted?)
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Tenascin C contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
プロトコール
参考文献
This product has been referenced in:
- Molina-Molina M et al. Anti-fibrotic effects of pirfenidone and rapamycin in primary IPF fibroblasts and human alveolar epithelial cells. BMC Pulm Med 18:63 (2018). Read more (PubMed: 29703175) »
- Valnegri P et al. RNF8/UBC13 ubiquitin signaling suppresses synapse formation in the mammalian brain. Nat Commun 8:1271 (2017). Read more (PubMed: 29097665) »
レビューと Q&A
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