Anti-Tenascin C 抗体 [EPR4219] (ab108930)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4219] to Tenascin C
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Tenascin C antibody [EPR4219]
Tenascin C 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4219] to Tenascin C -
由来種
Rabbit -
特異性
IHC on human tissues which we tested (such as testis, pancreas and stomach) showed non-specific staining. We don't recommend this antibody for IHC on human tissues.
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アプリケーション
適用あり: IHC-P, WB, IHC-Frmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-Fr: Mouse E14 spinal cord and cerebellar cortex tissue; Rat cerebellar cortex tissue. IHC-P: Rat cerebellar cortex tissue; Mouse E14 spinal cord and cerebellar cortex tissue. WB: U87-MG cell lysate; Postnatal mouse cerebellum lysate; Postnatal rat brain lysate; Human fetal brain lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4219 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108930の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (10) |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 241 kDa.
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IHC-Fr | (2) |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Predicted molecular weight: 241 kDa. |
IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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機能
Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. -
配列類似性
Belongs to the tenascin family.
Contains 15 EGF-like domains.
Contains 1 fibrinogen C-terminal domain.
Contains 15 fibronectin type-III domains. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 3371 Human
- Entrez Gene: 21923 Mouse
- Entrez Gene: 116640 Rat
- Omim: 187380 Human
- SwissProt: P24821 Human
- SwissProt: Q80YX1 Mouse
- Unigene: 143250 Human
- Unigene: 454219 Mouse
see all -
別名
- 150 225 antibody
- Cytotactin antibody
- Glioma associated extracellular matrix antigen antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA Buffer, pH 9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain. Staining on the molecular layer of rat cerebellar cortex (PMID: 1372043) is observed.
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Immunohistochemistry (Frozen sections) analysis of mouse E14 spinal cord labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.
Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247) is observed.
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All lanes : Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution
Lane 1 : Human fetal brain
Lane 2 : Human liver
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 241 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer and concentration: 5% NFDM /TBST.
Liver is negative control (PMID: 1717349). The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse E14 spinal cord tissue sections labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain.
Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247) is observed.
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Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution + U87-MG (human glioblastoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 241 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer and concentration: 5% NFDM /TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).
-
Immunohistochemistry (Frozen sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.
Positive staining on the molecular layer of rat cerebellar cortex (PMID: 1372043) is observed.
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Immunohistochemistry (Frozen sections) analysis of mouse E14 cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.
Positive staining on the molecular layer of mouse E14 cerebellar cortex (PMID: 1372043) is observed.
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All lanes : Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution
Lane 1 : Postnatal (P0) mouse cerebellum
Lane 2 : Postnatal (P0) rat brain
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 241 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure Time:
Lane 1: 15 seconds
Lane 2: 30 secondsThe molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).
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Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution + Human fetal brain lysate at 10 µg
Predicted band size: 241 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9 (ab93684). Hematoxylin was used to counterstain. A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Staining on the molecular layer of mouse cerebellar cortex (PMID: 1372043) is observed.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (81)
ab108930 は 81 報の論文で使用されています。
- Lu K et al. N-Acetyl-L-cysteine facilitates tendon repair and promotes the tenogenic differentiation of tendon stem/progenitor cells by enhancing the integrin α5/β1/PI3K/AKT signaling. BMC Mol Cell Biol 24:1 (2023). PubMed: 36604630
- Helms EJ et al. Mesenchymal Lineage Heterogeneity Underlies Nonredundant Functions of Pancreatic Cancer-Associated Fibroblasts. Cancer Discov 12:484-501 (2022). PubMed: 34548310
- Sterken BA et al. C/EBPβ isoform-specific regulation of migration and invasion in triple-negative breast cancer cells. NPJ Breast Cancer 8:11 (2022). PubMed: 35042889
- Zhang P et al. Tenascin-C can Serve as an Indicator for the Immunosuppressive Microenvironment of Diffuse Low-Grade Gliomas. Front Immunol 13:824586 (2022). PubMed: 35371015
- Rong W et al. Caspase-8 Promotes Pulmonary Hypertension by Activating Macrophage-Associated Inflammation and IL-1β (Interleukin 1β) Production. Arterioscler Thromb Vasc Biol 42:613-631 (2022). PubMed: 35387479