細胞タイプの特性評価

PSCを、神経細胞、心臓細胞、肺細胞など目的の細胞タイプに分化させた後は、細胞が未分化細胞マーカー（Oct4、KLF-4、c-Myc、Sox2など）を発現しなくなったことを確認することが重要です。 セクション2「PSCの維持」で述べた未分化細胞マーカーを使用して確認することができます。

また、この段階で、作製した細胞タイプが意図した細胞タイプとなっていることを確認することも重要です。 各細胞タイプでマーカーの存在を確認するために使用できる、おすすめの抗体を一部ご紹介します。

胚葉

中胚葉

Brachyury：Recombinant Anti-Brachyury / Bry antibody [EPR18113] (ab209665)

CD31: Recombinant Anti-CD31 antibody [EP3095] (ab134168)

CD34: Recombinant Anti-CD34 antibody [EP373Y] (ab81289)

BMP2: Recombinant Anti-BMP2 antibody [EPR20807] (ab214821)

BMP4: Recombinant Anti-BMP4 antibody [EPR6211] (ab124715)

N-cadherin：Anti-N Cadherin antibody (ab18203)

Nodal: Recombinant Anti-Nodal antibody [EPR2057] (ab109317)

Snail: Recombinant Anti-SNAIL antibody [EPR21043] (ab216347)

内胚葉

Sox17: Recombinant Anti-SOX17 antibody [EPR20684] (ab224637)

FOXA2: Recombinant Anti-FOXA2 antibody [EPR4466] (ab108422)

外胚葉

Nestin: Recombinant Anti-Nestin antibody [SP103] (ab105389)

Notch1: Recombinant Anti-Notch1 antibody [EP1238Y] (ab52627)

TP63: Recombinant Anti-p63 antibody [EPR5701] (ab124762)

神経細胞

神経幹細胞

Fas: Recombinant Anti-Fas antibody [EPR5700] (ab133619)

Frizzled-9：Recombinant Anti-Frizzled 9 antibody [EPR4011] (ab108628)

MSI1（Musashi-1）：Recombinant Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

MSl2（Musashi-2）：Recombinant Anti-MSI2 antibody [EP1305Y] (ab76148)

Nestin: Recombinant Anti-Nestin antibody [SP103] (ab105389)

Noggin：Recombinant Anti-Noggin antibody [EPR1561] (ab124977)

Pax-6：Recombinant Anti-PAX6 antibody [EPR15858] (ab195045)

Sox1: Recombinant Anti-SOX1 antibody [EPR4766] (ab109290)

Sox2: Recombinant Anti-SOX2 antibody [SP76] (ab93689)

神経細胞

α-Synuclein：Recombinant Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

NCAM：Recombinant Anti-NCAM1 antibody [EP2567Y] (ab75813)

Doublecortin：Recombinant Anti-Doublecortin antibody [EPR10935(B)] (ab167400)

GABA B Receptor：Recombinant Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)

GAP-43：Recombinant Anti-GAP43 antibody [EP890Y] (ab75810)

Glutamine Synthetase：Recombinant Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562)

MASH1：Recombinant Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

mGluR1α: Recombinant Anti-mGluR1a antibody [EPR13540] (ab183712)

Nestin: Recombinant Anti-Nestin antibody [SP103] (ab105389)

Nicastrin：Recombinant Anti-Nicastrin antibody [EPR2575Y] (ab68145)

Pax-5：Recombinant Anti-PAX5 antibody [EPR3730(2)] (ab109443)

P-glycoprotein：Recombinant Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

Synapsin I：Recombinant Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)

Synaptophysin：Recombinant Anti-Synaptophysin antibody [YE269] (ab32127)

アストロサイト

GFAP：Recombinant Anti-GFAP antibody [EPR1034Y] (ab68428)

EAAT1: Recombinant Anti-EAAT1 antibody [EPR12686] (ab181036)

EAAT2: Recombinant Anti-EAAT2 antibody [EPR19794] (ab205247)

Glutamine synthetase：Recombinant Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562)

S100β：Recombinant Anti-S100 beta antibody [EP1576Y] (ab52642)

ALDH1L1：Recombinant Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)

オリゴデンドロサイト

NG2: Recombinant Anti-NG2 antibody [EPR9195] (ab139406)

Olig1：Anti-Olig1 antibody [EPR6790] (ab124908)

オリゴデンドロサイト特異的タンパク質（OSP）：Recombinant Anti-Oligodendrocyte Specific Protein antibody [EPR12726] (ab175236)

Myelin basic protein (MBP)：Recombinant Anti-Myelin Basic Protein antibody [IGX3421] (ab209328)

Myelin oligodendrocyte glycoprotein (MOG)：Recombinant Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] (ab108505)

Sox10: Recombinant Anti-SOX10 antibody [EPR4007-104] (ab180862)

膵臓細胞

Glucagon：Recombinant Anti-Glucagon antibody [EP3070] (ab92517)

Insulin：Recombinant Anti-Insulin antibody [EPR17359] (ab181547)

Islet-1：Recombinant Anti-Islet 1 antibody [EP4182] (ab109517)

Pancreatic polypeptide：Recombinant Anti-Pancreatic Polypeptide antibody [EPR22853-61] (ab255827)

PDX-1：Recombinant Anti-PDX1 antibody [EPR3358(2)] (ab134150)

肝細胞

α-Fetoprotein：Recombinant Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)

Albumin：Recombinant Anti-Albumin antibody [EPSISR1] (ab137885)

HNF-1α：Recombinant Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)

Tat-SF1：Recombinant Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)

心臓細胞

α-Actinin：Recombinant Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Cardiac Troponin I：Recombinant Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)

Cardiac Troponin T：Recombinant Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)

Myogenin：Recombinant Anti-Myogenin antibody [EPR4789] (ab124800)

Nkx2.5: Recombinant Anti-Nkx2.5 antibody [EPR20168] (ab205263)