Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (chicken spinal cord section)
Permeabilization
Yes - Triton X (0.1%)
Specification
chicken spinal cord section
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST (0.1%) + 3% BSA
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluor® 568 Rabbit Anti-Mouse IgG (H+L)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
The primary antibody was titrated but even at the highest concentration used, we did not observe TDP43 immunoreactivity in sections that should have shown it.
Abcam response
Thank you for sharing this data with us. ab41881 has not previously been tested on Chicken or in IHC-Fr. However, based on this data it either does not work on this species, or in this application, or both, under these conditions. Abcam would welcome feedback from other customers using this product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 08 2013