Anti-TDP43 抗体 (ab41881)
製品の概要
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製品名Anti-TDP43 antibody
TDP43 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to TDP43
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由来種Rabbit
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アプリケーション適用あり: WB, IP, IHC-FoFrmore details
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種交差性交差種: Mouse, Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human TARDBP.
Immunogen の所有権に関して (Peptide available as ab41970.) -
ポジティブ･コントロール
- This antibody gave a positive signal in the following human lysates: HeLa Whole Cell, HeLa Nuclear, Jurkat Whole Cell, Jurkat Nuclear, HepG2 Whole Cell, HepG2 Nuclear, A431 Whole Cell and K562 Nuclear - tumor cell line.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab41881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa). | |
| IP | 1/50. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 20736350 |
ターゲット情報
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機能DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
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組織特異性Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
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関連疾患Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
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配列類似性Contains 2 RRM (RNA recognition motif) domains.
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ドメインThe RRM domains can bind to both DNA and RNA.
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翻訳後修飾Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. -
細胞内局在Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.
- Information by UniProt
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参照データベース
- Entrez Gene: 23435 Human
- Entrez Gene: 230908 Mouse
- Omim: 605078 Human
- SwissProt: Q13148 Human
- SwissProt: Q921F2 Mouse
- Unigene: 300624 Human
- Unigene: 635053 Human
- Unigene: 22453 Mouse
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別名
- ALS10 antibody
- OTTHUMP00000002171 antibody
- OTTHUMP00000002172 antibody
see all
画像
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-TDP43 antibody (ab41881)Image from Shan X et al, Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16325-30. Epub 2010 Aug 24, Fig 2. DOI 10.1073/pnas.1003459107ab41881 staining TARDBP in murine spinal cord by Immunohistochemistry (PFA perfusion fixed frozen sections).
Tissues fixed by 4% paraformaldehyde in phosphate buffer (pH 7.4) were cryoprotected by 30% sucrose in PBS (pH 7.4). Frozen sections (10 µm) of spinal cord tissues from three or more transgenic mice, respectively, were processed for immunofluorescence using ab41881. An Alexa Fluor 594 was used as secondary antibodies to visualize protein. Scale bar 20 µm.
TARDBP is localized in the nucleus and forms intranuclear granular structures, arrowheads. -
Western blot - Anti-TDP43 antibody (ab41881)Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: TDP43 knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (40 µg)
Lane 4: Jurkat cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab41881 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.ab41881 was shown to specifically react with TDP43 when TDP43 knockout samples were used. Wild-type and TDP43 knockout samples were subjected to SDS-PAGE. Ab41881 and ab8245 (loading control to GAPDH) were diluted at 1 µg/mL and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-TDP43 antibody (ab41881)All lanes : Anti-TDP43 antibody (ab41881) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 :Jurkat nuclear extract lysate (ab14844)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 :HepG2 nuclear extract lysate (ab14660)
Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 8 : K562 (Human) Nuclear Lysate - tumor cell line (ab29309)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted? -
Immunoprecipitation - Anti-TDP43 antibody (ab41881)This image is courtesy of an Abreview submitted by Dr Jayarama Krishnan BoseLane 1: Marker, Lane 2: 293T Total Lysate (Input), Lane 3: 293T supernatant (Flow through), LAne 4: Pull down (IP).
参考文献
This product has been referenced in:
- Li P et al. A liver-enriched long non-coding RNA, lncLSTR, regulates systemic lipid metabolism in mice. Cell Metab 21:455-67 (2015). Read more (PubMed: 25738460) »
- Huang CC et al. Metabolism and mis-metabolism of the neuropathological signature protein TDP-43. J Cell Sci 127:3024-38 (2014). Read more (PubMed: 24860144) »
