Anti-TCR V beta 5 抗体 [MEM-262] (ab1898)

Submit a review Q&A (2)References (3)

Key features and details

  • Mouse monoclonal [MEM-262] to TCR V beta 5
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-TCR V beta 5 antibody [MEM-262]

  • 製品の詳細

    Mouse monoclonal [MEM-262] to TCR V beta 5

  • 由来種

    Mouse

  • 特異性

    The antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

  • 種交差性

    交差種: Human

  • 免疫原

    Human thymoma cell line HPB-ALL.

  • 特記事項

    Functional Application Activates T cells (V beta 5-related subset).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MEM-262

  • アイソタイプ

    IgG2a

  • 研究分野

ターゲット情報

  • 関連性

    T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. The Vß5 TCR is a variant of the TCR ß chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vß5 has been reported to recognize some staphylococcus bacterial superantigens.

  • 細胞内局在

    Cell Membrane

  • 参照データベース

    • 別名

      • T cell receptor antibody
      • TCB antibody
      • TCRB antibody
      • TRB antibody
      • TRB@ antibody
      see all

    参考文献 (3)

    ab1898 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1898 は 3 報の論文で使用されています。

    • Wang CT  et al. Attenuating systemic inflammatory markers in simulated high-altitude exposure by heat shock protein 70-mediated hypobaric hypoxia preconditioning in rats. J Formos Med Assoc 114:328-38 (2015). PubMed: 25839766
    • Grabherr MG  et al. Exploiting nucleotide composition to engineer promoters. PLoS One 6:e20136 (2011). PubMed: 21625601
    • Pavlistová D  et al. A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor. Immunol Lett 88:105-8 (2003). PubMed: 12880678

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    Which lysates are recommended as positive controls for these antibodies?

    Read More
    Answer

    Thank you for your patience. For ab9219, the originator has recommended the following. Since the antibody recognizes human cytokeratin 18 we recommend using the PBS-insoluble fraction (pellet) of human cell lines containing cytokeratin 18 (for example Hela or T24, which was used for immunization of the mice) as a positive control in WB. You may also want to refer to the following publications for additional information about this antibody: Ramaekers F et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136:641-55 (1990). PubMed: 1690512 Schaafsma HE et al. Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am J Pathol 136:329-43 (1990). PubMed: 1689541 If you have any additional questions, please contact us again.

    Read More

    Question

    Which lysates are recommended as positive controls for these antibodies?

    Read More
    Answer

    Thank you for your enquiry. The originator of ab1898 was able to provide me with the following information. Western blot detection: -material: HPB-ALL cell line -non-reducing conditions -detects only free beta chains -molecular weight 36 kDa The following publication has used this antibody and you may find some additional here as well: Pavlistová D et al. A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor. Immunol Lett 88:105-8 (2003). PubMed: 12880678 I have not yet received information regarding ab9219 and will contact you again when I do.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com