Anti-TCR V beta 5 抗体 [MEM-262] (ab1898)

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Key features and details

  • Mouse monoclonal [MEM-262] to TCR V beta 5
  • Suitable for: Flow Cyt, IP, WB
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-TCR V beta 5 antibody [MEM-262]

  • 製品の詳細

    Mouse monoclonal [MEM-262] to TCR V beta 5

  • 由来種

    Mouse

  • 特異性

    The antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

  • アプリケーション

    適用あり: Flow Cyt, IP, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Human thymoma cell line HPB-ALL.

  • 特記事項

    Functional Application Activates T cells (V beta 5-related subset).

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MEM-262

  • アイソタイプ

    IgG2a

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.

ターゲット情報

  • 関連性

    T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. The Vß5 TCR is a variant of the TCR ß chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vß5 has been reported to recognize some staphylococcus bacterial superantigens.

  • 細胞内局在

    Cell Membrane

  • 参照データベース

    • 別名

      • T cell receptor antibody
      • TCB antibody
      • TCRB antibody
      • TRB antibody
      • TRB@ antibody
      see all

    参考文献

    ab1898 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1898 は 3 報の論文で使用されています。

    • Wang CT  et al. Attenuating systemic inflammatory markers in simulated high-altitude exposure by heat shock protein 70-mediated hypobaric hypoxia preconditioning in rats. J Formos Med Assoc 114:328-38 (2015). PubMed: 25839766
    • Grabherr MG  et al. Exploiting nucleotide composition to engineer promoters. PLoS One 6:e20136 (2011). PubMed: 21625601
    • Pavlistová D  et al. A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor. Immunol Lett 88:105-8 (2003). PubMed: 12880678

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Question

    Which lysates are recommended as positive controls for these antibodies?

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    Answer

    Thank you for your patience. For ab9219, the originator has recommended the following. Since the antibody recognizes human cytokeratin 18 we recommend using the PBS-insoluble fraction (pellet) of human cell lines containing cytokeratin 18 (for example Hela or T24, which was used for immunization of the mice) as a positive control in WB. You may also want to refer to the following publications for additional information about this antibody: Ramaekers F et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136:641-55 (1990). PubMed: 1690512 Schaafsma HE et al. Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am J Pathol 136:329-43 (1990). PubMed: 1689541 If you have any additional questions, please contact us again.

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    Question

    Which lysates are recommended as positive controls for these antibodies?

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    Answer

    Thank you for your enquiry. The originator of ab1898 was able to provide me with the following information. Western blot detection: -material: HPB-ALL cell line -non-reducing conditions -detects only free beta chains -molecular weight 36 kDa The following publication has used this antibody and you may find some additional here as well: Pavlistová D et al. A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor. Immunol Lett 88:105-8 (2003). PubMed: 12880678 I have not yet received information regarding ab9219 and will contact you again when I do.

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