Key features and details
- Rabbit polyclonal to TCR alpha
- Suitable for: IP, IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品名Anti-TCR alpha antibody
TCR alpha 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to TCR alpha
アプリケーション適用あり: IP, IHC-Fr, IHC-P, WBmore details
種交差性交差種: Mouse, Rat, Human
Recombinant full length protein (Human)
This product is manufactured by BioVision, an Abcam company and was previously called 5648 TCR alpha Antibody. 5648-100 is the same size as the 100 µg size of ab18861.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
バッファーPreservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
精製度Immunogen affinity purified
The Abpromise guarantee
IP: Use at a concentration of 10 - 20 µg/ml.
WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 16 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
関連性TCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma and delta chains. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR.
- T cell antigen receptor alpha polypeptide antibody
- T cell receptor alpha antibody
- T cell receptor alpha locus antibody
All lanes : Anti-TCR alpha antibody (ab18861) at 1 µg/ml
Lane 1 : Human thymus tissue lysate - total protein (ab30146)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa, 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
TCR alpha contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab18861 は 2 報の論文で使用されています。
- Liu Y et al. ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation. Cell Mol Immunol 18:1904-1919 (2021). PubMed: 32572163
- Liu Y et al. Neuronal IFN-beta-induced PI3K/Akt-FoxA1 signalling is essential for generation of FoxA1(+)Treg cells. Nat Commun 8:14709 (2017). IF ; Mouse . PubMed: 28436428