Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
HeLa cell line
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA+TTBS 1%
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 75 kDa Non-specific: several kDa
Positive control
Lane 1: HeLa cells treated with siRNA control
Lane 2: HeLa cells treated with siRNA against TCF25
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 25 2012