Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HEK 293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk/TBS/0.1% Tween 20
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti rabbit LgG HRP conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
40 second(s)
Bands
Specific: 79 kDa
Additional data
Additional Notes
Image description: both lanes are HEK293 whole cell lysates.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Gufa Lin
Verified customer
投稿 Jan 03 2020