Anti-Tbx3 抗体 (ab99302)
Key features and details
- Rabbit polyclonal to Tbx3
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Tbx3 antibody -
製品の詳細
Rabbit polyclonal to Tbx3 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details
適用なし: IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- This antibody gave a positive signal in nuclear extracts from the following cell lines: HepG2; MCF7. IHC-P (FFPE): Human Placenta (Normal) tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99302の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 5 µg/ml.
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| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). |
ターゲット情報
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機能
Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. -
組織特異性
Widely expressed. -
関連疾患
Defects in TBX3 are the cause of ulnar-mammary syndrome (UMS) [MIM:181450]. UMS is characterized by ulnar ray defects, obesity, hypogenitalism, delayed puberty, hypoplasia of nipples and apocrine glands. -
配列類似性
Contains 1 T-box DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 374202 Chicken
- Entrez Gene: 6926 Human
- Entrez Gene: 21386 Mouse
- Entrez Gene: 353305 Rat
- Omim: 601621 Human
- SwissProt: O15119 Human
- SwissProt: P70324 Mouse
- SwissProt: Q7TST9 Rat
see all -
別名
- Bladder cancer related protein XHL antibody
- T box 3 antibody
- T-box protein 3 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Tbx3 antibody (ab99302)
ab99302 staining Tbx3 in HepG2 cells. The cells were fixed with 4% Paraformaldehyde (10min), permeabilized with 0.1%PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab99302 at 5μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) pre-adsorbed, at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) pre-adsorbed, at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Perkin Elmer, Operetta CLS).
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Western blot - Anti-Tbx3 antibody (ab99302)All lanes : Anti-Tbx3 antibody (ab99302) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lane 2 :MCF7 nuclear extract lysate (ab14860)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 79 kDa
Additional bands at: 22 kDa, 77 kDa (possible isoform). We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Ab99302 should recognise all three isoforms of Tbx3. We are probably observing Isoform I and Isoform II on this Western Blot image. The molecular weights of Isoform I and Isoform II are 77- and 79-kDa respectively. -
Immunocytochemistry/ Immunofluorescence - Anti-Tbx3 antibody (ab99302)This image is courtesy of an Abreview by Zoe Burke.ab99302 staining Tbx3 in the AR42J-B13 rat pancreatic tumor cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton/PBS and blocked with blocking buffer for 60 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/5000.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab99302 は 21 報の論文で使用されています。
- Okawa ER et al. Essential roles of insulin and IGF-1 receptors during embryonic lineage development. Mol Metab 47:101164 (2021). PubMed: 33453419
- Ichijo R et al. Vasculature-driven stem cell population coordinates tissue scaling in dynamic organs. Sci Adv 7:N/A (2021). PubMed: 33568475
- Bhat-Nakshatri P et al. A single-cell atlas of the healthy breast tissues reveals clinically relevant clusters of breast epithelial cells. Cell Rep Med 2:100219 (2021). PubMed: 33763657
- Crane-Smith Z et al. A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development. Front Cell Dev Biol 9:595744 (2021). PubMed: 33869166
- Ramli MNB et al. Human Pluripotent Stem Cell-Derived Organoids as Models of Liver Disease. Gastroenterology 159:1471-1486.e12 (2020). PubMed: 32553762