Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Brain)
Specification
Embryonic Brain
Fixative
Formaldehyde
Permeabilization
Yes - Triton 0.5%
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + BSA 1%
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/800
Additional data
Additional Notes
This antibody worked the first time I used it. No need to make hundreds of tests to find the good conditions. Also works after heat-mediated antigen retrieval. I used PFA 4% for fixation overnight.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 19 2009