アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    tbr2--eomes-antibody-ab23345.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Cell Type Marker Neuron marker Soma marker
Share by email

Anti-TBR2 / Eomes 抗体 (ab23345)

  • Datasheet
  • SDS
Reviews (39)Q&A (28)References (403)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)
  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)
  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)
  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)
  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)
  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)
  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)
  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)

Key features and details

  • Rabbit polyclonal to TBR2 / Eomes
  • Suitable for: IHC-Fr, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-TBR2 / Eomes antibody
    TBR2 / Eomes 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to TBR2 / Eomes
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-Fr, WBmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Common marmoset
  • 免疫原

    Synthetic peptide corresponding to Mouse TBR2/ Eomes aa 650 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab25698)

  • ポジティブ・コントロール

    • IHC-Fr: C57/BL6 and Mash1 mouse forebrain coronal sections. Mouse brain E14 frozen tissue section. Mouse developing cerebral cortex tissue sections. Mouse embryonic brain tissue section. WB: E14 Mouse Embryo Brain Tissue Lysate. EL4 cells. Human PTA-6967 Whole Cell Lysate. Human ES Cells. Human Mesendoderm (Day 2) Whole Cell Lysate.
  • 特記事項

    Tbr2 expression is observed in neuron progenitor compartments in development (the subventricular zone and ventricular zone) and expression rises and falls with cortical plate neurogenesis. Transition from radial glia to intermediate progenitor cell is associated with upregulation of Tbr2.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 一次抗体 備考

    Tbr2 expression is observed in neuron progenitor compartments in development (the subventricular zone and ventricular zone) and expression rises and falls with cortical plate neurogenesis. Transition from radial glia to intermediate progenitor cell is associated with upregulation of Tbr2.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Developmental Biology
    • Reproduction
    • Germ cell markers
    • Developmental Biology
    • Lineage specification
    • Trophectoderm
    • Neuroscience
    • Development

関連製品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human TBR2 / Eomes protein (ab152851)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab23345の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr (10)
1/100 - 1/500.

Abcam recommends the following antigen retrieval method: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

We recommend using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody.

WB (5)
Use a concentration of 0.4 - 2.5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 72 kDa).

Abcam recommends using milk as the blocking agent.

In our hands, when tested in western blot, this product typically gives a weaker signal in mouse tissue lysates compared to human cell lines. However, this product gives clean, specific staining in IHC-Fr on mouse E14.5 cortex. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented.

特記事項
IHC-Fr
1/100 - 1/500.

Abcam recommends the following antigen retrieval method: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

We recommend using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody.

WB
Use a concentration of 0.4 - 2.5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 72 kDa).

Abcam recommends using milk as the blocking agent.

In our hands, when tested in western blot, this product typically gives a weaker signal in mouse tissue lysates compared to human cell lines. However, this product gives clean, specific staining in IHC-Fr on mouse E14.5 cortex. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented.

ターゲット情報

  • 機能

    Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.
  • 組織特異性

    Expressed in CD8+ T-cells.
  • 関連疾患

    Note=A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum.
  • 配列類似性

    Contains 1 T-box DNA-binding domain.
  • 発生段階

    Detected at 7 weeks of development in the forebrain floorplate of the CNS. Expressed within the mantle layer and migrating neuroblasts of the telencephalon at 12.5 weeks of development.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession O95936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 8320 Human
    • Entrez Gene: 13813 Mouse
    • Entrez Gene: 233242 Rat
    • Entrez Gene: 316052 Rat
    • Omim: 604615 Human
    • SwissProt: O95936 Human
    • SwissProt: O54839 Mouse
    • Unigene: 591663 Human
    • Unigene: 200692 Mouse
    see all
  • 別名

    • eomes antibody
    • EOMES_HUMAN antibody
    • Eomesodermin antibody
    • Eomesodermin homolog antibody
    • t box brain 2 antibody
    • T-box brain protein 2 antibody
    • T-brain-2 antibody
    • Tbr 2 antibody
    • TBR-2 antibody
    see all

画像

  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)
    Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)Image from Azim K et al., PLoS One. 2012;7(11):e49087. Fig 5.; doi: 10.1371/journal.pone.0049087. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Dlx2 and Tbr2 identify non overlapping progenitor lineages in the adult mouse SVZ (subventricular zone).

    (A–B) Adult C57/BL6 mouse forebrain coronal sections at rostro-caudal point 1.2 relative to the bregma were immunostained for Dcx (red), Dlx2 or Tbr2 (green) and Ki67 (blue). Both progenitor populations show characteristics of migrating neuroblasts, as indicated by their Dcx expression (C–D). Adult Mash1 mouse forebrain coronal sections at rostro-caudal point 1.2 relative to the bregma were immunostained for Tbr2 (red) and Dlx2 (blue). Both Tbr2 and Dlx2 exhibited EGFP expression, but showed no colocalisation. Right side captions show cropped individual channels and the merges. Full panel insets are zoomed and cropped DAB stained photomicrographs of rostral periventricular sections for Tbr2 in the dorsal SVZ (A), Dlx2 in the dorso-lateral SVZ (B) and Mash1 in the ventro-lateral SVZ (C). Yellow arrows and arrowheads show respectively positive stained cell and low level TF staining. Dotted lines mark approximate boundaries of ventricular space. Flattened confocal z-stacks are of 14–15 µm thickness, including captions. Scale bars: 15 µm in full panels, 20 µm in captions and 25 µm in insets.

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)
    Western blot - Anti-TBR2 / Eomes antibody (ab23345)
    All lanes : Anti-TBR2 / Eomes antibody (ab23345) at 1 µg/ml

    Lane 1 : Human PTA-6967 Whole Cell Lysate at 20 µg
    Lane 2 : E14 Mouse Embryo Brain Tissue Lysate at 40 µg

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 72 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes


    Gel type: MOPS

    Blocking buffer: 1% milk

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)
    Western blot - Anti-TBR2 / Eomes antibody (ab23345)
    ab23345 at 1 mg/ml + Human ES Cells treated with Retinoic Acid (48h) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 72 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?
    Additional bands at: 50 kDa, 65 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab23345 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Abcam recommends using milk as the blocking agent.

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)
    Western blot - Anti-TBR2 / Eomes antibody (ab23345)
    Anti-TBR2 / Eomes antibody (ab23345) at 1 µg/ml + Human ES Cells treated with Retinoic Acid (24h) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 72 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab23345 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Abcam recommends using milk as the blocking agent.

  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)
    Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)

    IHC image of TRB2 staining in a mouse brain E14 frozen tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). Non-specific protein-protein interactions were then blocked in TBS containing 0.2% (v/v) Triton X-100 for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.05% (v/v) Triton X-100 and 1% (w/v) BSA with ab23345 at 1/100 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody was used to detect the primary antibody.  The section was mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)
    Western blot - Anti-TBR2 / Eomes antibody (ab23345)
    All lanes : Anti-TBR2 / Eomes antibody (ab23345) at 1 µg/ml (BLOCKED WITH 3% MILK)

    Lane 1 : Human Mesendoderm (Day 2) Whole Cell Lysate
    Lane 2 : E14 Mouse Embryo Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 72 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)
    Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)This image is courtesy of an Abreview submitted by Dr Guillermo Estivill-Torrus

    ab23345 staining mouse developing cerebral cortex tissue sections by IHC-Fr.  Sections were PFA fixed and permeabilized in TX-100 prior to blocking with 2.5% serum for 1 hour at RT.  The primary antibody was diluted 1/500 and incubated with the sample for 18 hours.  A biotinylated pig anti-rabbit IgG antibody, diluted 1/500, was used as the secondary.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)
    Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)This image is a courtesy of Anonymous Abreview

    ab23345 staining TBR2 / Eomes in mouse embryonic brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocking with 1% BSA and normal Goat serum for 30 minutes at RT. The sample was incubated with primary antibody (1/1000 in TBS + BSA 1%) for 10 hours at 40C. An Alexa Fluor® 555-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/800 dilution.

    See Abreview

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)
    Western blot - Anti-TBR2 / Eomes antibody (ab23345)This image was submitted as part of a review published on 9th May 2006
    Lanes 1-3 : Anti-TBR2 / Eomes antibody (ab23345) at 1/2000 dilution
    Lanes 4-6 : V5 antibody

    Lane 1 : EL4 cells + empty vector
    Lane 2 : EL4 cells + vector expressing V5 tagged Eomesodermin
    Lanes 3 & 6 : EL4 cells + V5 tagged vector
    Lane 4 : EL4 cells + empty vector
    Lane 5 : EL4 cells + vector expressing V5 tagged Eomesodermin

    Secondary
    All lanes : Goat anti Rabbit at 1/2500 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa



    ab23345 detects a clear band of ~ 72 kDa in lysates from EL4 cells expressing V5 tagged Eomesodermin (lane 2).  Lanes 1 and 3 contain lysates from EL4 cells expressing empty vector or V5 tag alone. Lanes 4-6 show the same lysates blotted with anti-V5 tag antibody. GAPDH was used as a loading control.

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)
    Western blot - Anti-TBR2 / Eomes antibody (ab23345)
    All lanes : Anti-TBR2 / Eomes antibody (ab23345) at 1 µg/ml

    Lane 1 : Human Mesendoderm (Day 2) Whole Cell Lysate
    Lane 2 : Human Mesendoderm (Day 2) Whole Cell Lysate with Mouse TBR2 / Eomes peptide (ab25698) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 72 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?
    Additional bands at: 74 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345)
    Western blot - Anti-TBR2 / Eomes antibody (ab23345)This image is a courtesy of Anonymous Abreview
    Anti-TBR2 / Eomes antibody (ab23345) at 1/1000 dilution + Lysate prepared from mouse embryonic brain tissue at 20 µg

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 72 kDa
    Observed band size: 72 kDa


    Exposure time: 5 minutes

    See Abreview

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (403)

ab23345 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab23345 は 403 報の論文で使用されています。

  • Amadei G  et al. Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro. Dev Cell 56:366-382.e9 (2021). PubMed: 33378662
  • Caramello A  et al. Dentate gyrus development requires a cortical hem-derived astrocytic scaffold. Elife 10:N/A (2021). PubMed: 33393905
  • Zhang W  et al. PlGF knockdown attenuates hypoxia-induced stimulation of cell proliferation and glycolysis of lung adenocarcinoma through inhibiting Wnt/ß-catenin pathway. Cancer Cell Int 21:18 (2021). PubMed: 33407494
  • Mak KH  et al. Histone demethylase JMJD2B/KDM4B regulates transcriptional program via distinctive epigenetic targets and protein interactors for the maintenance of trophoblast stem cells. Sci Rep 11:884 (2021). PubMed: 33441614
  • Trujillo CA  et al. Pharmacological reversal of synaptic and network pathology in human MECP2-KO neurons and cortical organoids. EMBO Mol Med 13:e12523 (2021). PubMed: 33501759
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 67 Abreviews or Q&A

Immunocytochemistry abreview for Anti-TBR2 / Eomes antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (Embryonic stem cells)
Permeabilization
Yes - methanol
Specification
Embryonic stem cells
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Dec 15 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TBR2 / Eomes antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Common marmoset Tissue sections (Cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Cerebellum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Jul 27 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TBR2 / Eomes antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (e16 brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Specification
e16 brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 May 18 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TBR2 / Eomes antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (e14/15 brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Specification
e14/15 brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 May 03 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-TBR2 / Eomes antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (E13 brain tissue)
Permeabilization
Yes - 0.3% Triton X-100
Specification
E13 brain tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More

DR. Bhavin Shah

Verified customer

投稿 Feb 27 2018

Immunocytochemistry abreview for Anti-TBR2 / Eomes antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cell (Neural precursor cells)
Permeabilization
Yes - 0.3% triton
Specification
Neural precursor cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Read More

DR. Kate Hawkins

Verified customer

投稿 Aug 03 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-TBR2 / Eomes antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (ESC?)
Permeabilization
No
Specification
ESC?
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More

Naor Sagy

Verified customer

投稿 Jul 03 2017

Western blot abreview for Anti-TBR2 / Eomes antibody - ChIP Grade

Below Average
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (E14.5 forebrain)
Gel Running Conditions
Non-reduced Denaturing (4-20% TGX)
Loading amount
30 µg
Specification
E14.5 forebrain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Ms. Xiaofeng Zhou

Verified customer

投稿 May 22 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-TBR2 / Eomes antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kidney)
Permeabilization
Yes - 0.1% troton x-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Jun 20 2016

Western blot abreview for Anti-TBR2 / Eomes antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 May 04 2016

1-10 of 67 Abreviews or Q&A

  •  戻る
  • 1
  • 2
  • 3
  • 4
  • 5
  • ...
  • 次へ 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

技術情報や製品情報をメールで受け取る 登録
製品・研究分野別
  • 癌
  • 循環器
  • 細胞生物学
  • 発生
  • エピジェネティクス&核内情報
  • 免疫学
  • メタボリズム
  • 感染症
  • ニューロサイエンス
  • シグナル伝達
  • ステムセル
製品・タイプ別
  • 一次抗体
  • 二次抗体
  • バイオケミカルズ
  • アイソタイプ・コントロール
  • マルチカラー・セレクター
  • キット
  • ローディング・コントロール
  • ライゼート
  • ペプチド
  • タンパク質
  • スライド
  • タグ抗体・細胞マーカー
  • その他試薬
サービス&サポート
  • サポート
  • よくあるお問い合わせ
  • プロトコール&トラブルシュート
  • ご購入に関して
  • お問い合わせフォーム
  • トレーニング
  • ターゲット別に見る
アブカムについて
  • 企業ウェブサイト
  • 投資家の皆様へ
  • 企業情報
  • 採用情報
  • アブカムについて
イベント
  • 展示会
  • カンファレンス
International websites
  • abcam.com
  • abcam.cn

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
© 1998-2022 Abcam plc. All rights reserved.