Anti-TBLR1/TBL1XR1 抗体 [EPR16153] (ab190796)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16153] to TBLR1/TBL1XR1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TBLR1/TBL1XR1 antibody [EPR16153]
TBLR1/TBL1XR1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR16153] to TBLR1/TBL1XR1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- K562, Jurkat, Hela, Raw 264.7, C6, PC-12 and NIH-3T3 cell lysates; Human prostatic hyperplasia and mouse muscle tissue; HeLa and PC-3 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16153 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab190796の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/250.
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WB |
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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特記事項 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/250. |
WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
ターゲット情報
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機能
F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of the N-Cor corepressor complex that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of N-Cor complex, thereby allowing cofactor exchange, and transcription activation. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the WD repeat EBI family.
Contains 1 F-box-like domain.
Contains 1 LisH domain.
Contains 8 WD repeats. -
ドメイン
The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 79718 Human
- Entrez Gene: 81004 Mouse
- Entrez Gene: 365755 Rat
- Omim: 608628 Human
- SwissProt: Q9BZK7 Human
- SwissProt: Q8BHJ5 Mouse
- Unigene: 581171 Human
- Unigene: 202966 Mouse
see all -
別名
- C21 antibody
- DC42 antibody
- F box like/WD repeat containing protein TBL1XR1 antibody
see all
画像
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All lanes : Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa -
All lanes : Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw 264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostatic hyperplasia tissue, labeling TBLR1/TBL1XR1 with ab190796 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse muscle tissue, labeling TBLR1/TBL1XR1 with ab190796 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde, labeling TBLR1/TBL1XR1 with ab190796 at 1/200 dilution. Goat anti rabbit IgG(Alexa Fluor® 555) was used as the secondary antibody at 1/200. Counterstained with DAPI.
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Immunofluorescence analysis of PC-3 cells fixed in 4% paraformaldehyde, labeling TBLR1/TBL1XR1 with ab190796 at 1/200 dilution. Goat anti rabbit IgG(Alexa Fluor® 555) was used as a secondary antibody at 1/200. Counterstained with DAPI.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab190796 は 2 報の論文で使用されています。
- Becares N et al. Impaired LXRa Phosphorylation Attenuates Progression of Fatty Liver Disease. Cell Rep 26:984-995.e6 (2019). PubMed: 30673619
- Liu F et al. TBL1XR1 Is Highly Expressed in Gastric Cancer and Predicts Poor Prognosis. Dis Markers 2016:2436518 (0). PubMed: 27672238