Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (lung carcinoma)
Total protein in input
500 µg
Immuno-precipitation step
Protein G
Specification
lung carcinoma
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: RIPA buffer
Dilution
1/200
Additional data
Additional Notes
50% of the immunoprecipitated material was loaded on each lane. Membrane was blocked with blocking buffer (PBS+ 5% non fat milk powder) for 1 hour at room temperature followed by incubation with primary antibody (Tblr1) in blocking buffer + 0.1% Tween-20 overnight (around 16 hours) at 4 degree centigrade. Blot was washed thrice in PBS+0.1% Tween-20, followed by incubation with anti-rabbit IgG HRPO in blocking buffer + 0.1% Tween-20 at room temperature for 1 hour. Blot was washed thrice in PBS+0.1% Tween-20, followed by ECL detection of reactive bands.
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投稿 Jun 10 2011