製品の概要
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製品名Anti-TBL1 antibody
TBL1 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to TBL1
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由来種Goat
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特異性This antibody is also expected to recognise the human protein TBLR1 (NP_078941), which is very similar.
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アプリケーション適用あり: IHC, ELISA, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse
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免疫原
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特記事項Principal Names - TBL1X; transducin (beta)-like 1X-linked; EBI; TBL1; transducin (beta)-like 1; transducin (beta)-like 1 X-linked. Official Gene Symbol - TBL1X. GenBank Accession Number – NP_005638. LocusLink ID - 6907.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab2243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC | Use a concentration of 4 - 6 µg/ml. | |
| ELISA | Use at an assay dependent concentration. Antibody detection limit dilution 1:4,000. |
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| WB | Use a concentration of 3 - 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human TBL1 peptide (ab22881). |
ターゲット情報
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機能F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of corepressor complexes that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of transcription repressor complexes, thereby allowing cofactor exchange.
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組織特異性Ubiquitous.
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配列類似性Belongs to the WD repeat EBI family.
Contains 1 F-box-like domain.
Contains 1 LisH domain.
Contains 8 WD repeats. -
ドメインThe F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 6907 Human
- Entrez Gene: 21372 Mouse
- Omim: 300196 Human
- SwissProt: O60907 Human
- SwissProt: Q9QXE7 Mouse
- Unigene: 495656 Human
- Unigene: 258476 Mouse
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別名
- EBI antibody
- F box like/WD repeat protein TBL1X antibody
- F-box-like/WD repeat-containing protein TBL1X antibody
see all
画像
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Immunohistochemistry - Anti-TBL1 antibody (ab2243)Immunohistochemical analysis of paraffin embedded Human Kidney tissue labeling TBL1 with ab5642 at 4 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.
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Western blot - Anti-TBL1 antibody (ab2243)ab2243 staining (4µ g/ml) of Human Brain lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab2243 staining (4µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
参考文献
This product has been referenced in:
- Hoberg JE et al. SMRT derepression by the IkappaB kinase alpha: a prerequisite to NF-kappaB transcription and survival. Mol Cell 16:245-55 (2004). Read more (PubMed: 15494311) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"