製品の概要

  • 製品名
    Tau Research Antibody Panel
  • 製品の概要

    ab226492 is a tau research panel containing 1 recombinant mouse monoclonal antibody, 1 anti-mouse secondary antibody, 8 recombinant rabbit monoclonal antibodies and 1 anti-rabbit secondary antibody: Anti-Tau antibody, Goat Anti-Mouse IgG (H&L) (HRP), Anti-Tau (phospho S396) antibody, Anti-Tau (phospho T231) antibody, Anti-Tau (phospho S422) antibody, Anti-Tau (phospho S199) antibody, Anti-Tau (phospho S404) antibody, Anti-Tau (phospho S198) antibody, Anti-Tau (phospho S214) antibody, Anti-Tau (phospho S202 + T205) antibody, and Goat Anti-Rabbit IgG (H&L) (HRP).


    Under normal circumstances, tau is a microtubule-associated protein involved in microtubule stabilization, but under pathological conditions, tau becomes hyperphosphorylated and detaches from microtubules. Phosphorylated tau then aggregates to form paired helical filaments (PHFs) and neurofibrillary tangles (NFTs).


    The antibodies in this panel were selected for their exceptional performance. Please see the individual datasheets for additional information.

製品の特性

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  • ICC/IF image of ab80579 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80579, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab92676 staining Tau (phospho S404) in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/100).

  • Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling Tau (phospho S214) with ab170892 at 1/100 dilution.

  • ab79540, at 1/100 dilution, staining Tau (phospho S198) in paraffin-embedded human brain tissue by Immunohistochemistry.

  • IHC image of Tau (phospho T231) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with citrate buffer. The section was then incubated with ab151559 at 1/2000 dilution for 2 hours at 21ºC. A biotin conjugated goat-anti-rabbit antibody was used as a secondary at 1/250. The section shows clear neurofibrillary tangles in a subset of neurons.

  • IHC image of Tau (phospho S396) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with citrate buffer. The section was then incubated with unpurified ab109390 at 1/1000 dilution for 2 hoursat 21ºC. A biotin conjugated goat-anti-rabbit antibody was used as a secondary at 1/250. The section shows clear neurofibrillary tangles in a subset of neurons.

  • Dot blot analysis of Tau (phospho S202+ T205) labeled with ab210703 at 1/1000 dilution.

    Lane 1: Tau (phospho S202 + T205) peptide.
    Lane 2: Tau (phospho S202) peptide.
    Lane 3: Tau (phospho T205) peptide.
    Lane 4: Tau non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Dot blot analysis of Tau (pS199) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS199) with ab81268 at a dilution of 1/1000. ab97051 (peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Exposure time: 3 minutes.
    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot blot analysis of Tau (pS422) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS422) with ab79415 at a dilution of 1/1000. ab97051 (Peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

参考文献

ab226492 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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