Application
Flow Cytometry
Fixation
Methanol
Sample
Human Cell (SHSY5Y)
Specification
SHSY5Y
Gating Strategy
all living single cells were gated
Permeabilization
Yes - 0.5% Tween-20
Other product details
Incubation time
30 minute(s) · Temperature: 25°C · Diluent: 1%BSA with 0.1% Tween-20 in PBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Mouse AlexFluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 10 2015