製品の概要

  • 製品名
    Anti-Tau antibody [TAU-5] - BSA and Azide free
    Tau 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TAU-5] to Tau - BSA and Azide free
  • 由来種
    Mouse
  • アプリケーション
    適用あり: Flow Cyt, WB, IP, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Cow, Human
  • 免疫原

    Full length native protein (purified) corresponding to Cow Tau. Purified bovine microtubule-associated proteins.

  • ポジティブ・コントロール
    • IMR5 cells, human T98G glioblastoma cells or Alzheimer’s disease brain tissue.
  • 特記事項

    This antibody is without BSA and sodium azide.

    Abcam recommended secondaries - Goat Anti-Mouse HRP (ab205719) and Goat Anti-Mouse Alexa Fluor® 488 (ab150113).

    See other anti-mouse secondary antibodies that can be used with this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 79 kDa.
IP Use at 2 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration. PubMed: 24959311

ターゲット情報

  • 機能
    Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
  • 組織特異性
    Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
  • 関連疾患
    Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
    Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
    Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
    Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
    Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.
  • 配列類似性
    Contains 4 Tau/MAP repeats.
  • 発生段階
    Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
  • ドメイン
    The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
  • 翻訳後修飾
    Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
    Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
    Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
  • 細胞内局在
    Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    There are 9 isoforms produced by alternative splicing.
  • 別名
    • AI413597 antibody
    • AW045860 antibody
    • DDPAC antibody
    • FLJ31424 antibody
    • FTDP 17 antibody
    • G protein beta1/gamma2 subunit interacting factor 1 antibody
    • MAPT antibody
    • MAPTL antibody
    • MGC134287 antibody
    • MGC138549 antibody
    • MGC156663 antibody
    • Microtubule associated protein tau antibody
    • Microtubule associated protein tau isoform 4 antibody
    • Microtubule-associated protein tau antibody
    • MSTD antibody
    • Mtapt antibody
    • MTBT1 antibody
    • MTBT2 antibody
    • Neurofibrillary tangle protein antibody
    • Paired helical filament tau antibody
    • Paired helical filament-tau antibody
    • PHF tau antibody
    • PHF-tau antibody
    • PPND antibody
    • PPP1R103 antibody
    • Protein phosphatase 1, regulatory subunit 103 antibody
    • pTau antibody
    • RNPTAU antibody
    • TAU antibody
    • TAU_HUMAN antibody
    • Tauopathy and respiratory failure, included antibody
    see all

画像

  • ICC/IF image of ab80579 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80579, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Denatured IP using Human cells: IP 2µg/mg lysate. WB at 2µg/ml.

  • Overlay histogram showing SH-SY5Y cells stained with ab80579 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80579, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab80579 staining tau in SKNSH cells treated with prostaglandin J2 (ab120913), by ICC/IF. Expression of tau expression is restringed to the perinuclear zone with increased concentration of prostaglandin J2, as described in literature.
    The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab120913 (prostaglandin J2) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab80579 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei (blue) were counterstained with DAPI and membrane is was stained using WGA (red).

参考文献

This product has been referenced in:
  • Mahaman YAR  et al. Moringa Oleifera Alleviates Homocysteine-Induced Alzheimer's Disease-Like Pathology and Cognitive Impairments. J Alzheimers Dis 63:1141-1159 (2018). Read more (PubMed: 29710724) »
  • Fang YY  et al. Evidence of altered depression and dementia-related proteins in the brains of young rats after ovariectomy. J Neurochem 146:703-721 (2018). Read more (PubMed: 29939407) »
See all 38 Publications for this product

レビューと Q&A

1-8 of 8 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - nuclear (hypocampus)
Gel Running Conditions
Non-reduced Denaturing (NuPage Gels 4-12% BT gels))
Loading amount
20 µg
Specification
hypocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Aug 31 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus and cerebral cortex)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Hippocampus and cerebral cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Sergi Bayod

Verified customer

投稿 Sep 05 2016

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole fish)
Loading amount
10 µg
Specification
whole fish
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Nov 27 2015

Application
Flow Cytometry
Fixation
Methanol
Sample
Human Cell (SHSY5Y)
Specification
SHSY5Y
Gating Strategy
all living single cells were gated
Permeabilization
Yes - 0.5% Tween-20

Abcam user community

Verified customer

投稿 Feb 10 2015

Answer

Thank you very much for your reply.

I'm very sorry that we don't have more data about the new lot in IP. We don't have the older lots of the antibody in stock, but we do have a couple of new lots that you might want to try. If you'd like, I can send some of the newer antibody for you to try, or I can arrange a credit or refund for your purchase.

Please let me know if you would like to accept one of these options. I look forward to hearing from you.

Read More

Answer

Thank you again for letting us know about the recent trouble with this antibody and for your patience.

Each lot of this antibody is not tested for its binding affinity, so unfortunately this data is not available. Each new lot is tested in Western blot only, and I've asked the lab to review the QC data for this antibody. The WB results have been consistent with the newer lots, so there's no indication at this time that the affinity has changed. The IP and mass spec assay is probably more sensitive to changes in affinity compared to WB, which would explain why you've noticed changes that the lab has not. I am very sorry for the variability in performance and for the discrepancy.

Have you had a chance to try the assay again with the replacement vials that we sent? Please keep me updated with the results, and let me know if there is anything else that we can do for you.

Have a nice day.

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Answer

Thank you very much for your call today and for letting us know about the trouble with the recent lots of ab80579.

As we discussed, I'm sending 4 free of charge vials of ab80579 on the order ***, which should arrive on Tuesday of next week.

If you could send the protocol that you use for the IP, as well as any supporting data, I'll forward this to the lab so they can look into the issue. I will also request QC data for this recent lot and ask if there have been any noticeable changes in affinity.

I look forward to hearing from you. Please let me know if you have further questions or need anything else, and I'll be happy to help.

Read More

Answer


I just got confirmation from our lab that this WB was done after an IP experiment. Therefore, the lower band is probably just a another mouse IgG heavy chain fragment (a left over from the prior IP), which has a weight of 50 kDa.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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