Application
Flow Cytometry
Sample
Human Cell (SHSY5Y stably transfected with Cas9)
Permeabilization
Yes - 0.5% Tween-20
Gating Strategy
single cells
Specification
SHSY5Y stably transfected with Cas9
Preparation
Cell harvesting/tissue preparation method: 0.05% Trypsin to lift cells, Wash one time with PBS
Sample buffer: PBS
Sample buffer: PBS
Fixation
Paraformaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor blocking buffer
Dilution
1/80
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
gRNAs were introduced by lentivirus under U6 promoter.
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投稿 Aug 19 2016