Application
Western blot
Sample
Human Cell lysate - whole cell (SHSY5Y)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Loading amount
10 µg
Specification
SHSY5Y
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Detection
Detection method
Licor
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Positive control
4RTau cDNA overexpression
Additional data
Additional Notes
4RTau is the 0N4R isoform of full length Tau protein.
SHSY5Y endogenous Tau is mainly 0N3R Tau (band slightly lower than 4R Tau)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 03 2016