Anti-Tau 抗体 (ab62639)
Key features and details
- Sheep polyclonal to Tau
- Suitable for: IHC-Fr, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Tau antibody
Tau 一次抗体 製品一覧 -
製品の詳細
Sheep polyclonal to Tau -
由来種
Sheep -
特異性
The specificity of this antibody refers to P10636-8.
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アプリケーション
適用あり: IHC-Fr, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human Tau aa 1-100.
Database link: P10636-8 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.02% Thimerosal (merthiolate)
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62639の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr | ||
IHC-P | (1) | |
ICC/IF |
特記事項 |
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IHC-P: 1/4000 - 1/8000.
IHC-Fr: 1/4000 - 1/8000.
This antibody achieves excellent staining of Tau aggregations within neurons (tangles), and distrophic neurons within plaques and also stains Tau threads within axons.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. -
組織特異性
Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system. -
関連疾患
Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613. -
配列類似性
Contains 4 Tau/MAP repeats. -
発生段階
Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain. -
ドメイン
The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats. -
翻訳後修飾
Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD. -
細胞内局在
Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components. - Information by UniProt
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参照データベース
- Entrez Gene: 450177 Chimpanzee
- Entrez Gene: 4137 Human
- Omim: 157140 Human
- SwissProt: Q5YCW1 Chimpanzee
- SwissProt: P10636 Human
- Unigene: 101174 Human
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製品の状態
There are 9 isoforms produced by alternative splicing. -
別名
- AI413597 antibody
- AW045860 antibody
- DDPAC antibody
see all
画像
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IHC-P image of Tau staining on Human colon tissue section using ab62639 (1/4000). The section were subjected to heat mediated antigen retrieval using citric acid (pH 6). The sections were then blocked using 1% BSA for 10 min at 21°C. The primary antibody was incubated for 2 hours at 21°C.
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ab62639 at 1/400 dilution staining Tau in neurons by confocal Immunohistochemistry. Jackson Cy2 secondary antibody (1/100).
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ab62639 at 1/400 dilution staining Tau in dystrophic neurites within plaques by confocal Immunohistochemistry. Jackson Cy2 secondary antibody (1/100).
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ab62639 at 1/8000 staining Tau in neurons within neuronal axons Tau threads (as indicated by arrows), by Light Immunohistochemsitry. Jackson biotinylated donkey anti rabbit secondary antibody (1/2000) followed by Sigma Extra avidin peroxidase (1/400) and DAB substrate.
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ab62639 at 1/8000 staining Tau in dystrophic neurites within plaques, within neuronal axons Tau threads (as indicated by arrows), by Light Immunohistochemsitry. Jackson biotinylated donkey anti rabbit secondary antibody (1/2000) followed by Sigma Extra avidin peroxidase (1/400) and DAB substrate.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab62639 は 2 報の論文で使用されています。
- Jiang J et al. Intraneuronal sortilin aggregation relative to granulovacuolar degeneration, tau pathogenesis and sorfra plaque formation in human hippocampal formation. Front Aging Neurosci 14:926904 (2022). PubMed: 35978952
- Saito T et al. Humanization of the entire murine Mapt gene provides a murine model of pathological human tau propagation. J Biol Chem 294:12754-12765 (2019). PubMed: 31273083