Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Rat Cell lysate - nuclear (airway epithelial cell)
Specification
airway epithelial cell
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Detection
Detection method
ECL
Bands
Specific: 40 kDa Non-specific: 30, 55 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
Rat cytoplasmic lysate
Positive control
Rat nuclear lysate
Additional data
Additional Notes
Lane 1 = magic mark
Lane 2= Empty
Lanes 3-8 = rat airway epithelial cell nuclear lysate
Lane 9 = rat airway epithelial cell cytoplasmic lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Joseph Cichocki
Verified customer
投稿 Mar 07 2014