GFAP

GeneName

GFAP

Summary

GFAP, or glial fibrillary acidic protein, is a 50 kDa intermediate filament protein primarily expressed in astrocytes, where it plays a pivotal role in maintaining the structural integrity of the cytoskeleton. It is localised in various cellular components, including the cytoplasm, cell body, and astrocyte end-feet. GFAP is involved in several biological processes, such as astrocyte development, extracellular matrix organisation, and the regulation of neurotransmitter uptake. Additionally, it contributes to neuron projection regeneration and the differentiation of Bergmann glial cells, highlighting its importance in both astrocyte function and neuronal support.

Importance

GFAP is relevant to: - Neurodegenerative diseases, as its expression is often upregulated in response to central nervous system injury or disease, serving as a marker for astrogliosis. - The study of astrocyte function and their role in synaptic plasticity, which is crucial for understanding learning and memory. - Research into the mechanisms of neuroinflammation, given its involvement in the regulation of inflammatory responses in the brain. - Developmental biology, particularly in the context of glial cell differentiation and function in the nervous system.

Top Products

For researchers investigating GFAP, we recommend two excellent primary antibodies that cater to a variety of applications. The first is the highly regarded polyclonal antibody, Anti-GFAP antibody - Astrocyte Marker (ab7260), which has garnered an impressive 1532 citations, underscoring its reliability in the field. This antibody is particularly effective for immunohistochemistry (IHC), immunocytochemistry (ICC), western blotting (WB), and immunoprecipitation (IP).In addition, we offer the recombinant antibody, Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab68428). This product has been validated for use in IHC, ICC, WB, flow cytometry (FC), and IP, making it a versatile choice for researchers seeking consistent performance across multiple applications. With 168 citations, it is also gaining recognition within the research community. Together, these antibodies provide robust options for studying GFAP in various experimental contexts. The Human GFAP Matched Antibody Pair Kit (ab222279), supported by 6 citations, is an excellent option for researchers looking to accurately measure GFAP levels in their samples.

Abcam Product Citation Summary

The data indicates a significant focus on GFAP in various contexts related to neurobiology, particularly in studies involving astrocyte activation, neuroinflammation, and neurogenesis. The use of GFAP antibodies spans multiple species, predominantly Mus musculus and Rattus norvegicus, highlighting its relevance in both basic and translational research. Applications include immunohistochemistry (IHC), immunofluorescence (IF), and western blotting (WB), suggesting a comprehensive approach to studying GFAP's role in different neurological conditions and responses.