CA9
GeneName
CA9
Summary
CA9, also known as CAIX or G250, is a 50 kDa transmembrane protein that is primarily expressed in the basolateral plasma membrane of epithelial cells, particularly in the kidney and certain tumours. It functions as a carbonic anhydrase, catalysing the reversible conversion of carbon dioxide and water to bicarbonate and protons. This enzyme activity plays a critical role in maintaining acid-base balance and facilitating bicarbonate transport. CA9 is involved in various biological processes, including morphogenesis of an epithelium and responses to hypoxia and testosterone, and is also implicated in the secretion of substances in response to xenobiotic stimuli. Its presence in the nucleolus and cell projections suggests additional regulatory roles in cellular functions.
Importance
CA9 is relevant to: - Cancer biology, particularly in renal cell carcinoma, where it is often overexpressed and serves as a potential biomarker for diagnosis and prognosis - The study of hypoxia in tumours, as it is upregulated in response to low oxygen levels, influencing tumour adaptation and survival - Understanding acid-base homeostasis in the kidney, contributing to the regulation of pH in renal physiology - Investigating the effects of testosterone and xenobiotics on epithelial cell function and behaviour
Top Products
For researchers investigating CA9, we recommend two excellent primary antibodies that cater to various experimental needs. The first is the well-cited polyclonal antibody, Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086), which has garnered 148 citations, highlighting its reliability in the field. This antibody is suitable for immunohistochemistry (IHC), western blotting (WB), and immunocytochemistry (ICC), making it a versatile choice for your research.Additionally, we offer the recombinant antibody, Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] (ab108351), which has been validated in knockout models. This monoclonal antibody is ideal for IHC and WB applications and has 20 citations, indicating its growing acceptance among researchers. The recombinant nature of this product ensures batch-to-batch consistency, providing confidence in your experimental results. The Anti-Carbonic Anhydrase 9/CA9 antibody ELISA Kit (ab105226) is an excellent option for researchers looking to study CA9 in their experiments.
Abcam Product Citation Summary
The data indicates that CA9 is being studied in various human cancer contexts, including colorectal cancer, lung cancer, and soft-tissue sarcomas. The use of both Western blotting and immunohistochemistry suggests a focus on protein expression levels and localisation in different tumour types, highlighting the relevance of CA9 in cancer biology and potential therapeutic implications.
Abcam Product Citation Table
Function
Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
Post-translational modifications
Asn-346 bears high-mannose type glycan structures.
Sequence Similarities
Belongs to the alpha-carbonic anhydrase family.
Tissue Specificity
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Cellular localization
- Nucleus
- Nucleus
- Nucleolus
- Cell membrane
- Single-pass type I membrane protein
- Cell projection
- Microvillus membrane
- Single-pass type I membrane protein
- Found on the surface microvilli and in the nucleus, particularly in nucleolus.
Alternative names
G250, MN, CA9, Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CAIX, RCC-associated antigen G250
Database links
swissprot:Q16790 entrezGene:768 omim:603179
Other research areas
- Immuno-oncology