BCL2L11
GeneName
BCL2L11
Summary
BCL2L11, also known as BIM or Bod, is a 22 kDa pro-apoptotic member of the Bcl-2 protein family. It is primarily localised to the mitochondrial outer membrane and is involved in the intrinsic apoptotic signalling pathway, particularly in response to cellular stress and DNA damage. BCL2L11 interacts with other Bcl-2 family proteins to regulate mitochondrial membrane permeability and promote the release of cytochrome c, a key step in apoptosis. Additionally, it plays roles in various biological processes including B cell homeostasis, developmental pigmentation, and organ growth, reflecting its involvement in both cell survival and death mechanisms.
Importance
BCL2L11 is relevant to: - Cancer research, as it influences apoptosis and can affect tumour progression and response to therapy - Autoimmune diseases, where its role in T cell homeostasis is critical for immune regulation - Developmental biology, given its involvement in processes such as embryonic digit morphogenesis and organ development - Neurodegenerative disorders, due to its function in neuronal apoptosis and stress responses
Top Products
For researchers investigating BCL2L11, we highly recommend the top-selling recombinant antibody, Anti-Bim antibody [Y36] (ab32158). This antibody has been validated in knockout models, ensuring reliable performance in various applications, including Western blotting (WB), immunohistochemistry (IHC), flow cytometry (FC), immunocytochemistry (ICC), and immunoprecipitation (IP). With 120 citations, it is well-regarded in the research community, making it an excellent choice for those studying BCL2L11.
Abcam Product Citation Summary
The data indicates that BCL2L11 is primarily studied in the context of apoptosis and cell proliferation across various human and mouse cell lines and tissues. The use of both Western blotting and immunohistochemistry suggests a focus on understanding the protein's role in different biological processes, including mitochondrial pathway competency and the mechanisms of apoptosis.
Abcam Product Citation Table
Domain
The BH3 motif is required for interaction with Bcl-2 proteins and cytotoxicity.
Function
Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.
Post-translational modifications
Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation (PubMed:15486195, PubMed:21478148). Deubiquitination catalyzed by USP27X stabilizes the protein (By similarity).
Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein.
Sequence Similarities
Belongs to the Bcl-2 family.
Tissue Specificity
Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are widely expressed with tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
Cellular localization
- Endomembrane system
- Peripheral membrane protein
- Associated with intracytoplasmic membranes.
- Isoform BimEL
- Mitochondrion
- Translocates from microtubules to mitochondria on loss of cell adherence.
- Isoform BimL
- Mitochondrion
- Isoform BimS
- Mitochondrion
- Isoform Bim-alpha1
- Mitochondrion
Alternative names
BIM, BCL2L11, Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death