Anti-TARC/CCL17 抗体 (ab182793)
Key features and details
- Rabbit polyclonal to TARC/CCL17
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TARC/CCL17 antibody
TARC/CCL17 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TARC/CCL17 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human TARC/CCL17 aa 50 to the C-terminus.
Database link: Q92583 -
ポジティブ・コントロール
- WB: HepG2 cell, 293T cell, Human cerebrum tissue, Mouse kidney tissue lysates; IHC: Human liver cancer tissue.
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特記事項
This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. The product of this gene binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
(without Mg2+ and Ca2+) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab182793の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (4) |
1/50 - 1/200.
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WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 11 kDa.
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特記事項 |
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IHC-P
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 11 kDa. |
ターゲット情報
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機能
Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4. -
組織特異性
Expressed at high levels in thymus and at low levels in the lung, colon and small intestine. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 6361 Human
- Entrez Gene: 20295 Mouse
- Omim: 601520 Human
- SwissProt: Q92583 Human
- Unigene: 546294 Human
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別名
- A-152E5.3 antibody
- A152E53 antibody
- ABCD 2 antibody
see all
画像
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Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling TARC/CCL17 with ab182793 at 1/55.
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All lanes : Anti-TARC/CCL17 antibody (ab182793)
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Human cerebrum tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti rabbit IgG at 1/5000 dilution
Predicted band size: 11 kDaGel: 12% SDS-PAGE
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ab182793 staining TARC/CCL17 in human lung biopsy tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was performed by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/2000 in PBS + 1% BSA) for 16 hours at 4°C. A Biotin-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
TARC/CCL17 in connective tissue is revealed in brown (DAB impact). Nucleus (and Mucus) are counterstain in blue (Fastblue).
Fixative: Formalin 4% (Qpath), lungs were post-fixed (6h) before paraffin embedded in a tissue-tek® VIP6 automat.
Sections: 3µm on SuperFrost®.
Antigen retrieval step: Citrate buffer pH=6 (15 min at 95°C) in a water Bath then slide were placed in Sequenza immunostaining with Coverplate (Shandon).
Endogenous enzyme were blocked with Dual Endogenous Enzyme Block (Dako cytomation) 15min.
Extravidin-HRP was used to increase signal (1/500, 20min, RT).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab182793 は 14 報の論文で使用されています。
- Jo WS et al. Low Dose Rate Radiation Regulates M2-like Macrophages in an Allergic Airway Inflammation Mouse Model. Dose Response 20:15593258221117349 (2022). PubMed: 36003321
- Fu X et al. Qi-Long-Tian formula extract alleviates symptoms of acute high-altitude diseases via suppressing the inflammation responses in rat. Respir Res 22:52 (2021). PubMed: 33579290
- Deng S et al. Recombinant CCL17-dependent CCR4 activation alleviates neuroinflammation and neuronal apoptosis through the PI3K/AKT/Foxo1 signaling pathway after ICH in mice. J Neuroinflammation 18:62 (2021). PubMed: 33648537
- Zhang A et al. Lactate-induced M2 polarization of tumor-associated macrophages promotes the invasion of pituitary adenoma by secreting CCL17. Theranostics 11:3839-3852 (2021). PubMed: 33664865
- Yang J et al. Circular RNA CHST15 Sponges miR-155-5p and miR-194-5p to Promote the Immune Escape of Lung Cancer Cells Mediated by PD-L1. Front Oncol 11:595609 (2021). PubMed: 33777742